用于诊断来自脑脊液和无菌部位组织感染的非靶向宏基因组学方案。
Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites.
作者信息
Atkinson Laura, Lee Jack Cd, Lennon Alexander, Shah Divya, Storey Nathaniel, Morfopoulou Sofia, Harris Kathryn A, Breuer Judy, Brown Julianne R
机构信息
Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.
Department of Infection, Immunity and Inflammation, Institute of Child Health, University College London, London, UK.
出版信息
Heliyon. 2023 Sep 9;9(9):e19854. doi: 10.1016/j.heliyon.2023.e19854. eCollection 2023 Sep.
Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in our clinical service, processing 178 specimens over the past 2.5 years in a laboratory that adheres to ISO:15189 standards. We have successfully used this protocol to diagnose multiple cases of encephalitis and hepatitis.
宏基因组下一代测序（mNGS）是一种非靶向技术，能够检测样本中的所有微生物核酸。本方案概述了我们用于脑脊液（CSF）标本和来自无菌部位的组织进行mNGS的湿实验室方法。我们在临床服务中常规使用此方法，在一个符合ISO:15189标准的实验室中，在过去2.5年里处理了178个标本。我们已成功使用该方案诊断了多例脑炎和肝炎病例。
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