用于诊断来自脑脊液和无菌部位组织感染的非靶向宏基因组学方案。

Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites.

作者信息

Atkinson Laura, Lee Jack Cd, Lennon Alexander, Shah Divya, Storey Nathaniel, Morfopoulou Sofia, Harris Kathryn A, Breuer Judy, Brown Julianne R

机构信息

Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.

Department of Infection, Immunity and Inflammation, Institute of Child Health, University College London, London, UK.

出版信息

Heliyon. 2023 Sep 9;9(9):e19854. doi: 10.1016/j.heliyon.2023.e19854. eCollection 2023 Sep.

Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in our clinical service, processing 178 specimens over the past 2.5 years in a laboratory that adheres to ISO:15189 standards. We have successfully used this protocol to diagnose multiple cases of encephalitis and hepatitis.