Fibriani Azzania, Taharuddin Audrey Angelina Putri, Stephanie Rebecca, Yamahoki Nicholas, Laurelia Jessica, Wisnuwardhani Popi Hadi, Agustiyanti Dian Fitria, Angelina Marissa, Rubiyana Yana, Ningrum Ratih Asmana, Wardiana Andri, Iskandar Ferry, Permatasari Fitri Aulia, Giri-Rachman Ernawati Arifin
School of Life Sciences and Technology, Bandung Institute of Technology, Bandung, 40132, Indonesia.
Research Center for Nanoscience and Nanotechnology, Bandung Institute of Technology, Bandung, 40132, Indonesia.
Heliyon. 2023 Sep 12;9(9):e20089. doi: 10.1016/j.heliyon.2023.e20089. eCollection 2023 Sep.
Even entering the third year of the COVID-19 pandemic, only a small number of COVID-19 antiviral drugs are approved. Curcumin has previously shown antiviral activity against SARS-CoV-2 nucleocapsid, but its poor bioavailability limits its clinical uses. Utilizing nanotechnology structures, curcumin-derived carbon-dots (cur-CDs) were synthesized to increase low bioavailability of curcumin. analyses were performed using molecular docking, inhibition of SARS-CoV-2 nucleocapsid C-terminal domain (N-CTD) and antiviral activity were assessed in dimer-based screening system (DBSS) and respectively. Curcumin bound with the N-CTD at ΔG = -7.6 kcal/mol, however modifications into cur-CDs significantly improved the binding affinity and %interaction. Cur-CDs also significantly increased protection against SARS-CoV-2 in both DBSS and at MOI = 0.1. This study demonstrated the effect of post-infection treatment of curcumin and novel curcumin-derived carbon-dots on SARS-CoV-2 N-CTD dimerization. Further investigation on pre-infection and treatment of curcumin and cur-CDs are required for a comprehensive understanding on the carbon-dots enhanced antiviral activity of curcumin against SARS-CoV-2.
即使进入新冠疫情的第三年，获批的新冠抗病毒药物仍寥寥无几。姜黄素此前已显示出对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）核衣壳的抗病毒活性，但其较差的生物利用度限制了其临床应用。利用纳米技术结构，合成了姜黄素衍生的碳点（cur-CDs）以提高姜黄素较低的生物利用度。使用分子对接进行分析，在基于二聚体的筛选系统（DBSS）中评估对SARS-CoV-2核衣壳C末端结构域（N-CTD）的抑制作用和抗病毒活性。姜黄素与N-CTD结合时的自由能变化（ΔG）为-7.6千卡/摩尔，然而将其修饰为cur-CDs显著提高了结合亲和力和相互作用百分比。在DBSS和感染复数（MOI）=0.1时，cur-CDs在两者中也显著增强了对SARS-CoV-2的防护作用。本研究证明了感染后姜黄素及新型姜黄素衍生碳点对SARS-CoV-2 N-CTD二聚化的治疗效果。为全面了解碳点增强姜黄素对SARS-CoV-2的抗病毒活性，需要对姜黄素和cur-CDs的感染前及感染后治疗进行进一步研究。
