Acaricidal assessment of the fungal extract of against : Role of and analysis.

The mushroom, commonly referred to as the oyster mushroom, is a widely consumed edible mushroom that grows in clusters on dead or dying trees. In addition to its culinary uses, research has found that the mushroom may also have potential medicinal properties. The current study investigated the potential use of a fungal extract from as a natural acaricide against , a major vector of economically significant infections and one of the most significant bovine ectoparasites. The study used the adult immersion test (AIT) and the larvae packet test (LPT) to evaluate the effectiveness of the extract against ticks. To evaluate the reproductive effect of the fungal extract on the reproduction of engorged females, the eggs were tested for weight and egg-laying index. The results of the study showed that extract had a significant acaricidal effect, with a 40 mg/mL concentration causing 89 ± 2.64% mortality in larvae compared to the 91.00 ± 3.60% mortality by the positive control at 48 h interval. Whereas in the AIT, the extract inhibited 39.86 ± 4.13% of oviposition. The study also used approach to further examine the binding mechanisms of the compounds in the fungal extract to the target protein Triosephosphate isomerase RmTIM, using molecular docking in AutoDock Vina software. Docking scores of -8.3, -7.7 and -6.9 kcal/mol, respectively, showed that rutin, naringin and myricitin had significant interactions with the active site residues of the target protein. Our results suggest that extract may be a potential alternative to traditional acaricides for controlling in livestock.