Shafir M, Martinelli G, Bekesi J G, Jaramillo S, Heimann T, Aufses A H
Eur J Surg Oncol. 1986 Dec;12(4):351-7.
Chronically inflamed tissues and most malignancies have antigenic properties. Patients with long-standing inflammatory bowel disease (IBD) are prone to development of colorectal cancer, which is known to shed antigens in the bloodstream. In an effort to study immunological aspects of these diseases, sera from patients with IBD and colorectal cancer were evaluated for presence of circulating antigen-antibody immune complexes (CIC), and compared to normal controls. CIC were precipitated by polyethylene glycol (PEG) and found to be elevated in all diseased groups. Dissociation of CIC and quantification of their antibody component revealed significant elevation of each immunoglobulin in IBD and in patients with colorectal cancer versus control: IgG = 1.776 +/- 1.573 vs 0.734 +/- 0.618 (P less than 0.001); IgA = 0.368 +/- 0.452 vs 0.090 +/- 0.198 (P less than 0.001); IgM = 0.434 +/- 0.235 vs 0.080 +/- 0.285 (P less than 0.001) serum total Ig levels were consistently much higher than CIC Ig. No correlation was found between the individual serum Ig components and the precipitable complexes-bound Ig, suggesting a selective recognition of antigenic components in the CIC, rather than non-specific association and subsequent precipitation of serum Ig. CIC may represent an easily accessible source of immunological determinants for the study of malignant and chronic inflammatory diseases.
慢性炎症组织和大多数恶性肿瘤都具有抗原特性。患有长期炎症性肠病（IBD）的患者易患结直肠癌，已知结直肠癌会在血液中释放抗原。为了研究这些疾病的免疫学方面，对IBD患者和结直肠癌患者的血清进行了循环抗原 - 抗体免疫复合物（CIC）的检测，并与正常对照组进行比较。CIC通过聚乙二醇（PEG）沉淀，发现在所有患病组中均升高。CIC的解离及其抗体成分的定量显示，与对照组相比，IBD患者和结直肠癌患者的每种免疫球蛋白均显著升高：IgG = 1.776±1.573对0.734±0.618（P小于0.001）；IgA = 0.368±0.452对0.090±0.198（P小于0.001）；IgM = 0.434±0.235对0.080±0.285（P小于0.001），血清总Ig水平始终远高于CIC Ig。未发现个体血清Ig成分与可沉淀复合物结合的Ig之间存在相关性，这表明CIC中对抗原成分的选择性识别，而非血清Ig的非特异性结合及随后的沉淀。CIC可能是研究恶性和慢性炎症性疾病的免疫决定因素的一个易于获取的来源。
