Idström J P, Subramanian V H, Chance B, Scherstén T, Bylund-Fellenius A C
Fed Proc. 1986 Dec;45(13):2937-41.
The regulation of the energy metabolism in contracting skeletal muscle is under close control, and several regulating factors have been reported. The aim of this study was to investigate the importance of the oxygen supply as a limiting factor for muscle performance during contractions and recovery from contractions. To perform well-controlled standardized experiments on contracting skeletal muscle, the perfused rat hind limb model was developed. The 31P NMR technique was adapted to the rat hind limb model. This enabled continuous nondestructive monitoring of the energy state at various levels of muscular activity. Significant correlations were found between oxygen delivery and oxygen consumption, lactate release, and glucose uptake, respectively. An increased degree of fatigue was observed at lower oxygen deliveries. In both soleus and gastrocnemius muscles, oxygen delivery correlated with the intramuscular concentrations of phosphocreatine (PCr), lactate, and glycogen. The 31P NMR experiments showed a correlation between oxygen delivery and the steady-state level of PCr/inorganic phosphate (Pi) during the contraction period. The rate of recovery in PCr/Pi after the contraction was also dependent on oxygen delivery. The results demonstrate a causal relationship between oxygen supply and energy state in contracting as well as recovering skeletal muscles.
收缩骨骼肌中能量代谢的调节受到密切控制，并且已经报道了几种调节因子。本研究的目的是调查氧气供应作为收缩期间以及收缩恢复过程中肌肉性能限制因素的重要性。为了在收缩骨骼肌上进行严格控制的标准化实验，开发了灌注大鼠后肢模型。将31P核磁共振技术应用于大鼠后肢模型。这使得能够在不同肌肉活动水平下对能量状态进行连续无损监测。分别在氧气输送与氧气消耗、乳酸释放和葡萄糖摄取之间发现了显著相关性。在较低的氧气输送量下观察到疲劳程度增加。在比目鱼肌和腓肠肌中，氧气输送与肌内磷酸肌酸（PCr）、乳酸和糖原的浓度相关。31P核磁共振实验表明，在收缩期氧气输送与PCr/无机磷酸盐（Pi）的稳态水平之间存在相关性。收缩后PCr/Pi的恢复速率也取决于氧气输送。结果表明，在收缩和恢复的骨骼肌中，氧气供应与能量状态之间存在因果关系。
