Gussoni M, Giuliani A M, Ripamonti A, Boicelli C A
NMR Research Laboratories, San Raffaele Institute, Milano, Italy.
Physiol Chem Phys Med NMR. 1990;22(4):233-40.
The time dependence of Lactate (Lac), H+, Adenosine-triphosphate (ATP), Phosphocreatine (PCr), Hexose-monophosphate (PME), and Inorganic Phosphate (Pi) levels has been obtained for frog muscle at rest in anaerobic conditions by multinuclear NMR. All information has been collected on the same samples alternatively tuning the probehead on 1H- and 31P-NMR frequencies. ATP, PCr and H+ levels show the same time dependence for all the samples, while PME, Pi and Lac levels vary in time differently from one sample to another. No direct correlation between the Lac appearance and the H+ concentration has been found.
通过多核核磁共振技术，已获得了处于厌氧条件下的青蛙静止肌肉中乳酸（Lac）、氢离子（H⁺）、三磷酸腺苷（ATP）、磷酸肌酸（PCr）、磷酸己糖（PME）和无机磷酸盐（Pi）水平随时间的变化情况。所有信息均采集自相同样本，通过交替将探头调谐到¹H - 和³¹P - 核磁共振频率来实现。所有样本中，ATP、PCr和H⁺水平呈现相同的时间依赖性，而PME、Pi和Lac水平随时间的变化在不同样本间存在差异。未发现Lac出现与H⁺浓度之间存在直接关联。
