Jiang Xiaoxue, Lu Yi, Xie Sijie, Chen Yingji, Liu Xinlei, Li Shujie, Song Shuting, Wang Liyan, Lu Dongdong
Shanghai Putuo People's Hospital, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Departments of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Noncoding RNA Res. 2023 Sep 24;8(4):641-644. doi: 10.1016/j.ncrna.2023.09.005. eCollection 2023 Dec.
miRNA is a noncoding RNA found in recent years and more than one third of human genes are the target of miRNAs. miR-624, located on human chromosome 14, is associated with tumorigenesis. However, the role of miR-624 in human hepatocarcinogenesis is still unclear. Herein, our results indicate that miR-624 accelerates the growth of liver cancer cells Moreover, the modification distribution of H3K9me1 on chromosomes is different between rLV group and rLV-miR-624 group. miR-624 affects epigenetic regulation of several genes in human liver cancer cells, such as RAB21, SMARCD3, MAPK6,PRRX1, ZFHX3, EMC3 (TMEM111). Furthermore, miR-624 affects transcriptome of some genes in liver cancer, including RAB21， UBE2N， PPP1CC，KPNA3， RAB7A，CPEB2,KLF4， MARK2， JUN， ARF6， TMEM39A. On the other hand, miR-624 affects proteome of several genes in liver cancer, such as, RBM5,PTK2, KDM2A,POLR2H, POLR2G,CDK6,KIF15,CUL2,FKBP2,ErbB-3,JUN, PKM2, CyclinE,PLK1, mTOR, PPAR, Rab7A,ARAF, UPF3B ，PTEN, SUZ12, GADD45, H3.3, CUL5, ARF6,EMC3,ATG4B,ATG14,CALR. Interestingly, miR-624 affects the RAB7A interaction network in liver cancer cells, involving in CLTC，ITGB1，HNRNPU， DARS1， RPS16， CTPS1，H3-3B，JUN,MYH10， CUL5， CPSF7. Strikingly, excessive MEC3 abrogates the carcinogenic functions of miR-624. Importantly, our findings indicate that miR-624 affects some signaling pathway in liver cancer, including Wnt signaling pathway，Hippo signaling pathway，mTOR signaling pathway, Ras signaling pathway，MAPK signaling pathway，PI3K-Akt signaling pathway, erbB signaling pathway. These results provide a basis for the treatment of human liver cancer.
微小RNA（miRNA）是近年来发现的一种非编码RNA，超过三分之一的人类基因是miRNA的作用靶点。位于人类14号染色体上的miR-624与肿瘤发生有关。然而，miR-624在人类肝癌发生中的作用仍不清楚。在此，我们的结果表明，miR-624可促进肝癌细胞的生长。此外，rLV组和rLV-miR-624组染色体上H3K9me1的修饰分布不同。miR-624影响人类肝癌细胞中多个基因的表观遗传调控，如RAB21、SMARCD3、MAPK6、PRRX1、ZFHX3、EMC3（TMEM111）。此外，miR-624影响肝癌中一些基因的转录组，包括RAB21、UBE2N、PPP1CC、KPNA3、RAB7A、CPEB2、KLF4、MARK2、JUN、ARF6、TMEM39A。另一方面，miR-624影响肝癌中多个基因的蛋白质组，如RBM5、PTK2、KDM2A、POLR2H、POLR2G、CDK6、KIF15、CUL2、FKBP2、ErbB-3、JUN、PKM2、细胞周期蛋白E、PLK1、mTOR、PPAR、Rab7A、ARAF、UPF3B、PTEN、SUZ12、GADD45、H3.3、CUL5、ARF6、EMC3、ATG4B、ATG14、CALR。有趣的是，miR-624影响肝癌细胞中的RAB7A相互作用网络，涉及CLTC、ITGB1、HNRNPU、DARS1、RPS16、CTPS1、H3-3B、JUN、MYH10、CUL5、CPSF7。引人注目的是，过量的EMC3可消除miR-624的致癌功能。重要的是，我们的研究结果表明，miR-624影响肝癌中的一些信号通路，包括Wnt信号通路、Hippo信号通路、mTOR信号通路、Ras信号通路、MAPK信号通路、PI3K-Akt信号通路、erbB信号通路。这些结果为人类肝癌的治疗提供了依据。
