Khosravi Maryam, Shirani Amir Mansour, Ostadsharif Maryam, Saravani Ramin
Department of Oral Medicine, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Department of Medical Basic Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Dent Res J (Isfahan). 2023 Aug 28;20:89. eCollection 2023.
This study aimed to compare the effects of laser photodynamic therapy (PDT) with methylene blue (MB) or aminolevulinic acid (ALA) on the oral squamous cell carcinoma (OSCC) cell line.
In this experimental study, the C152 (KB) OSCC cell line was cultured in a culture medium containing 10% fetal bovine serum. The cells were exposed to 0.1, 0.2, 0.5, 1, 2, 5, and 10 mM concentrations of MB and ALA alone and combined with diode laser irradiation with 660 nm wavelength, 40 mW power, and 10 J/cm energy density in continuous-wave mode perpendicular to the surface. Cell viability was assessed using the methyl thiazolyl tetrazolium assay and compared among the groups by the Kruskal-Wallis test.
The results showed that the reduction in cell viability in the MB + laser and ALA + laser groups was greater than that in the MB and ALA groups without laser ( < 0.001). Significant differences were noted in cell viability in the presence of some different concentrations of MB and ALA ( < 0.05), such that by an increase in their concentration, cell viability decreased. Cell viability in the MB + laser group was significantly lower than that in the ALA + laser group in some photosensitizer concentrations ( < 0.05).
Within the limitations of this study, the results showed that laser PDT with MB (high concentrations) was more effective than laser PDT with ALA against the OSCC cell line.
本研究旨在比较激光光动力疗法（PDT）联合亚甲蓝（MB）或氨基乙酰丙酸（ALA）对口腔鳞状细胞癌（OSCC）细胞系的影响。
在本实验研究中，C152（KB）OSCC细胞系在含有10%胎牛血清的培养基中培养。细胞分别单独暴露于0.1、0.2、0.5、1、2、5和10 mM浓度的MB和ALA，并与波长660 nm、功率40 mW、能量密度10 J/cm²的二极管激光垂直于表面以连续波模式照射相结合。使用甲基噻唑基四氮唑法评估细胞活力，并通过Kruskal-Wallis检验在各组之间进行比较。
结果显示，MB+激光组和ALA+激光组的细胞活力降低幅度大于无激光照射的MB组和ALA组（P<0.001）。在存在一些不同浓度的MB和ALA时，细胞活力存在显著差异（P<0.05），即随着其浓度增加，细胞活力降低。在某些光敏剂浓度下，MB+激光组的细胞活力显著低于ALA+激光组（P<0.05）。
在本研究的局限性范围内，结果表明，高浓度MB的激光光动力疗法比ALA的激光光动力疗法对OSCC细胞系更有效。
