Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, PR China.
Department of Orthopaedics, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, PR China.
Colloids Surf B Biointerfaces. 2023 Nov;231:113570. doi: 10.1016/j.colsurfb.2023.113570. Epub 2023 Sep 27.
Chronic and infectious wounds are major public health issues with financial and clinical manifestations. Developing a multitasking extracellular matrix mimicking scaffold can bring revolution saving millions of lives. Many bioactive agents are offering therapeutic promises in managing infectious wounds but require a suitable delivery system to ensure not only their bioavailability possible on the wound site but also control their burst release hence making them either useless or highly cytotoxic. In this study, we reported the dual bioactive agent-loaded electrospinning nanofibers potentially useable against infectious wounds. The zinc oxide nanoparticles (ZnO NPs) and vascular endothelial growth factors (VEGF), highly relevant bioactive agents, were chosen to be co-delivered to the wound site through the core-shell electrospun membrane. The physicochemical properties of prepared membranes were characterized through various physicochemical tools. Our result demonstrated that PLGA polymer can be electrospun into smooth fibers. X-ray diffraction analysis revealed the successful loading of ZnO NPs which was further confirmed by TEM. The fabricated membrane exhibited a suitable mechanical behavior. Moreover, the incorporation of ZnO NPs has turned the nanofibers into an effective antibacterial scaffold. Besides, the membranes were also evaluated for their cytotoxicity. The in vitro cell culturing on various membranes revealed that cell maintained their maximum viability on all the membranes. The potential of in vivo wound healing was further demonstrated through animal experiments. Our results show that membranes could not only influence early wound contraction, but also better tissue organization demonstrated through histopathological evaluation. We successfully demonstrated the rich vascularization network by synching the actions of ZnO NPs and VEGF. In conclusion, the fabricated membranes possess suitable physicochemical properties and promising biological activity and hence should be further exploited for in vivo wound healing potential.
慢性和感染性伤口是具有财务和临床表现的主要公共卫生问题。开发一种多功能的细胞外基质模拟支架可以带来革命性的变化，挽救数百万人的生命。许多生物活性物质在管理感染性伤口方面具有治疗潜力，但需要合适的输送系统来确保不仅在伤口部位保持其生物利用度，而且还能控制其爆发式释放，从而避免它们变得无用或具有高细胞毒性。在本研究中，我们报道了双生物活性药物负载的静电纺丝纳米纤维，这种纳米纤维可能可用于治疗感染性伤口。选择氧化锌纳米颗粒（ZnO NPs）和血管内皮生长因子（VEGF）作为具有代表性的生物活性物质，通过核壳型静电纺丝膜共递送到伤口部位。通过各种物理化学工具对制备的膜的物理化学性质进行了表征。我们的结果表明，PLGA 聚合物可以被静电纺成光滑的纤维。X 射线衍射分析表明 ZnO NPs 的成功加载，这一结果通过 TEM 进一步得到证实。所制备的膜表现出适当的机械性能。此外，ZnO NPs 的掺入使纳米纤维成为一种有效的抗菌支架。此外，还对膜进行了细胞毒性评估。在各种膜上进行的体外细胞培养表明，细胞在所有膜上都保持最大的活力。通过动物实验进一步证明了其体内伤口愈合的潜力。我们的结果表明，膜不仅可以影响早期伤口收缩，而且通过组织学评估还可以更好地组织化。我们通过 ZnO NPs 和 VEGF 的协同作用成功地证明了丰富的血管化网络。总之，所制备的膜具有合适的物理化学性质和有前途的生物学活性，因此应该进一步开发用于体内伤口愈合的潜力。
