Knox P, Uphill P F, Fry J R, Benford J, Balls M
Food Chem Toxicol. 1986 Jun-Jul;24(6-7):457-63. doi: 10.1016/0278-6915(86)90092-x.
An in vitro method for assessing the relative acute toxicities of chemicals was developed through the collaboration of four tissue culture laboratories. Cytotoxicity was measured by using a dye-binding method to determine inhibition of the proliferation of BCL-D1 cells 72 hr after addition of the test chemical. A blind trial with 50 chemicals showed that the test procedure gave results with a high degree of interlaboratory reproducibility.
四个组织培养实验室合作开发了一种评估化学品相对急性毒性的体外方法。通过使用染料结合法测定添加测试化学品72小时后BCL-D1细胞增殖的抑制情况来测量细胞毒性。对50种化学品进行的盲法试验表明，该测试程序得出的结果具有高度的实验室间可重复性。
