Balls M, Horner S A
Food Chem Toxicol. 1985 Feb;23(2):209-13. doi: 10.1016/0278-6915(85)90019-5.
FRAME (the Fund for the Replacement of Animals in Medical Experiments) has established a research programme in collaboration with four research centres and a number of industrial companies, to determine whether cell cultures can reliably be used to replace live animal procedures in routine tests on the relative acute toxicities of chemicals. Following the development of the test protocol, initial trials were carried out to establish intralaboratory and interlaboratory reproducibility and the criteria for choice of cell types, end-points and metabolizing systems. A set of coded chemicals is being collected for use in blind trials and summaries of existing knowledge of the in vivo toxicity of these compounds are being produced. More recently, FRAME has established an international validation scheme for in vitro alternative methods.
FRAME（医学实验动物替代基金会）与四个研究中心及多家工业公司合作开展了一项研究计划，以确定在化学品相对急性毒性的常规测试中，细胞培养能否可靠地用于替代活体动物实验。在制定测试方案后，进行了初步试验，以确定实验室内和实验室间的可重复性以及细胞类型、终点和代谢系统的选择标准。正在收集一组编码化学品用于盲法试验，并正在编写这些化合物体内毒性的现有知识总结。最近，FRAME建立了体外替代方法的国际验证计划。
