College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China.
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China; Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, PR China; Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan 430070, PR China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, PR China.
J Hazard Mater. 2024 Jan 5;461:132631. doi: 10.1016/j.jhazmat.2023.132631. Epub 2023 Sep 26.
Microcystis aeruginosa and ammonia pollution are two important environmental stress factors in water eutrophication. Herein, we simulated environmental conditions to investigate the effects of chronic exposure (single and combined) to M. aeruginosa and total ammonia nitrogen (TAN) on lipid metabolism and muscle quality in zebrafish. Our results showed that M. aeruginosa and TAN significantly induced lipid deposition and tissue damage in the liver of zebrafish. Liver transcriptomic analysis revealed that M. aeruginosa and TAN disrupted the balance in lipid synthesis, decomposition, and transport, ultimately leading to hepatic lipid accumulation. Moreover, exposure to M. aeruginosa or TAN alone resulted in decreased crude protein content and increased lipid content in muscle, as well as disrupted muscle fatty acid composition. Metabolomic analysis of muscle revealed significant alterations in metabolites such as glycerolipids, glycerophospholipids and fatty acids. The co-exposure of M. aeruginosa and TAN had a more significant effect on liver lipid dysfunction and muscle quality deterioration in zebrafish. These findings provide valuable insights into the potential risks and hazards of M. aeruginosa and TAN in eutrophic water bodies subject to Microcystis blooms, and can help inform effective strategies for monitoring and managing these toxins in aquatic ecosystems.
铜绿微囊藻和氨氮污染是水体富营养化中两个重要的环境胁迫因素。本研究模拟环境条件，探讨了铜绿微囊藻和总氨氮（TAN）慢性暴露（单一和联合）对斑马鱼脂质代谢和肌肉品质的影响。结果表明，铜绿微囊藻和 TAN 显著诱导了斑马鱼肝脏的脂质沉积和组织损伤。肝脏转录组分析显示，铜绿微囊藻和 TAN 破坏了脂质合成、分解和转运的平衡，最终导致肝脏脂质积累。此外，单独暴露于铜绿微囊藻或 TAN 会导致肌肉中粗蛋白含量降低、脂质含量增加，以及肌肉脂肪酸组成紊乱。肌肉代谢组学分析显示，甘油磷脂、甘油磷酸脂和脂肪酸等代谢物发生了显著变化。铜绿微囊藻和 TAN 的共暴露对斑马鱼肝脂质功能障碍和肌肉品质恶化的影响更为显著。这些发现为富营养化水体中铜绿微囊藻和 TAN 的潜在风险和危害提供了有价值的见解，并有助于为水生生态系统中这些毒素的监测和管理提供有效的策略。
