Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of Korea.
Division of Sports Science, Kyonggi University, Suwon 16227, Republic of Korea.
FEMS Microbiol Lett. 2023 Jan 17;370. doi: 10.1093/femsle/fnad105.
We assessed the salt tolerance and proteolytic activity of 40 genome-published Bacillus subtilis strains isolated from fermented Korean foods to illuminate the genomic background behind the functionality of B. subtilis in high-salt fermentation. On the basis of the salt tolerance and phenotypic proteolytic activity of the 40 strains, we selected five strains exhibiting different phenotypic characteristics. Comparative genomic analyses of these five strains provided genomic insight into the salt tolerance and proteolytic activity of B. subtilis. Two-component system (TCS) genes annotated as ybdGJK and laterally acquired authentic ATP-binding cassette (ABC) transporter system genes of tandem repeat structure might contribute to increase salt tolerance. The additional possession of gene homologs for CAAX protease family proteins and components of Clp (caseinolytic protease) complex, ATP-dependent Clp proteolytic subunit ClpP and AAA+ (ATPases associated with diverse cellular Activities) family ATPases, might determine the proteolytic activity of B. subtilis. This study established the scientific foundation for the viability and functionality of B. subtilis in high-salt fermentation.
我们评估了 40 株从发酵韩国食品中分离的已发布基因组枯草芽孢杆菌菌株的耐盐性和蛋白水解活性，以阐明枯草芽孢杆菌在高盐发酵中功能的基因组背景。基于这 40 株菌的耐盐性和表型蛋白水解活性，我们选择了 5 株表现出不同表型特征的菌株。对这 5 株菌的比较基因组分析为枯草芽孢杆菌的耐盐性和蛋白水解活性提供了基因组见解。被注释为 ybdGJK 的双组分系统（TCS）基因和串联重复结构的侧向获得的真正 ATP 结合盒（ABC）转运体系统基因可能有助于提高耐盐性。额外拥有 CAAX 蛋白酶家族蛋白和 Clp（酪蛋白水解蛋白酶）复合物、ATP 依赖性 Clp 蛋白酶亚基 ClpP 和 AAA+（与多种细胞活动相关的 ATPases）家族 ATPases 的基因同源物可能决定了枯草芽孢杆菌的蛋白水解活性。这项研究为枯草芽孢杆菌在高盐发酵中的生存能力和功能奠定了科学基础。
