Werling H O, Spiess E, Paweletz N
Invasion Metastasis. 1986;6(5):257-69.
The highly metastatic and nonmetastatic variants of the rat tumor BSp73 have been tested for motility in vitro by a phagokinetic assay. Surprisingly, the nonmetastatic variant is locomotional, whereas the highly metastatic variant exhibited stationary motility only. Soluble factors of normal rat serum and medium conditioned by regenerating normal rat lung, or solid constituents of the extracellular matrix material which was exudated by normal rat lung fibroblasts or bovine cornea endothelial cells as well as collagen type III could not stimulate locomotory ability in these tumor cells. However, by contact to rat lung cells they acquired passive mobility. The significance of these cellular properties for their metastatic behavior is discussed.
利用吞噬动力学测定法，对大鼠肿瘤BSp73的高转移性和非转移性变体进行了体外运动能力测试。令人惊讶的是，非转移性变体具有运动能力，而高转移性变体仅表现出静止运动。正常大鼠血清的可溶性因子、由正常大鼠再生肺调节的培养基、正常大鼠肺成纤维细胞或牛角膜内皮细胞渗出的细胞外基质材料的固体成分以及III型胶原均不能刺激这些肿瘤细胞的运动能力。然而，通过与大鼠肺细胞接触，它们获得了被动运动性。讨论了这些细胞特性对其转移行为的意义。
