Hamada J
Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1987 Jul;62(4):652-60.
Three highly metastatic and two weakly metastatic clones were obtained from a spontaneously arising mammary adenocarcinoma in an SHR rat. The difference in their capacity to metastasize in lungs was recognized only when the cancer cells were inoculated subcutaneously but not when they were inoculated intravenously. This evidence possibly indicates that the difference in the metastatic capacity of these clones is caused by different potential for detachment from the primary site and for intravasation during the various steps of metastasis. The motility of cancer cells, which is one of the most important factors in these steps of metastasis, showed no difference between the highly and weakly metastatic clones. However, the motility of cancer cells was decreased after the coculture with normal fibroblasts, and the motility of weakly metastatic clones used was more strongly decreased than that of highly metastatic clones. On the other hand, using a dye transfer method to examine the relationship between the metastatic capacity of cancer cells and the capacity of cancer cells to make junctional communications with normal fibroblasts, it was demonstrated that the frequency of communication between weakly metastatic clone cells and fibroblasts was significantly higher than that between highly metastatic clone cells and fibroblasts. These results suggest that the motility of cancer cells is inhibited by interaction with normal fibroblasts, and that one of these forms of interaction may be mediated by intercellular communication.
从一只自发性乳腺腺癌的SHR大鼠中获得了三个高转移性克隆和两个低转移性克隆。只有当癌细胞皮下接种时，才能识别出它们在肺部转移能力的差异，而静脉接种时则无法识别。这一证据可能表明，这些克隆转移能力的差异是由转移各个步骤中从原发部位脱离和进入血管的不同潜力造成的。癌细胞的运动性是转移这些步骤中最重要的因素之一，在高转移性克隆和低转移性克隆之间没有差异。然而，与正常成纤维细胞共培养后，癌细胞的运动性降低，且所用低转移性克隆的运动性比高转移性克隆的运动性下降得更明显。另一方面，使用染料转移法研究癌细胞转移能力与癌细胞与正常成纤维细胞进行连接通讯能力之间的关系，结果表明低转移性克隆细胞与成纤维细胞之间的通讯频率明显高于高转移性克隆细胞与成纤维细胞之间的通讯频率。这些结果表明，癌细胞的运动性受到与正常成纤维细胞相互作用的抑制，且这些相互作用形式之一可能是由细胞间通讯介导的。
