Curtis S K, Cowden R R, Benner D B
Histochemistry. 1986;85(6):475-81. doi: 10.1007/BF00508429.
Seven basic fluorochromes with varying specificities were used to stain the large squamous epithelial cells isolated from the larval salivary glands of Megaselia scalaris (Phoridae). Although the EDTA-based method selected for isolating the cells produced permeabilization and a loss of viability of the cells, consistent results were obtained with the various fluorochromes. The "classical" pattern of green nuclear and red cytoplasmic fluorescence observed in cells stained with acridine orange could be changed to green cytoplasmic and red nuclear fluorescence by pretreatment with RNase. The predominantly cytoplasmic and nucleolar fluorescence obtained with pyronine Y could be changed to mainly nuclear fluorescence by RNase pretreatment. The other five fluorochromes tested were not affected appreciably by extraction with RNase. Quinacrine mustard, dicarbocyanine (DiOC3(3)), and rhodamine 123 produced primarily cytoplasmic and nucleolar fluorescence, while nile red revealed mainly cytoplasmic lipid droplets. Phosphine 3R initially stained lipid droplets but very rapidly redistributed throughout the cytoplasm and nucleus. Because of their large size, flatness, and content of histochemically demonstrable components, the cells of Megaselia are especially appropriate for use as "optical objects" or controls in various studies. New methods of isolating the cells, however, will be needed to prevent permeabilization and loss of viability of the cells.
使用七种具有不同特异性的基本荧光染料对从黑腹果蝇（蚤蝇科）幼虫唾液腺中分离出的大鳞状上皮细胞进行染色。尽管所选的基于乙二胺四乙酸（EDTA）的细胞分离方法导致细胞透化并丧失活力，但使用各种荧光染料仍获得了一致的结果。用吖啶橙染色的细胞中观察到的绿色细胞核和红色细胞质荧光的“经典”模式可通过用核糖核酸酶（RNase）预处理而变为绿色细胞质和红色细胞核荧光。用派洛宁Y获得的主要为细胞质和核仁荧光可通过RNase预处理变为主要为细胞核荧光。测试的其他五种荧光染料不受RNase提取的明显影响。喹吖因氮芥、双羰花青（DiOC3(3)）和罗丹明123主要产生细胞质和核仁荧光，而尼罗红主要显示细胞质脂滴。磷化3R最初对脂滴染色，但很快重新分布到整个细胞质和细胞核中。由于黑腹果蝇的细胞体积大、扁平且含有可通过组织化学方法证实的成分，因此特别适合用作各种研究中的“光学对象”或对照。然而，需要新的细胞分离方法来防止细胞透化和活力丧失。
