Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd. , Tokyo, Japan.
Department of Gastroenterology and Hepatology, Yokohama City University School of Medicine , Yokohama, Japan.
Microbiol Spectr. 2023 Dec 12;11(6):e0512322. doi: 10.1128/spectrum.05123-22. Epub 2023 Oct 11.
is one of the predominant oral bacteria in humans. However, this bacterium is enriched in colorectal cancer (CRC) tissues and may be involved in CRC development. Our previous research suggested that is present in CRC tissues originating from the oral cavity using a traditional strain-typing method [arbitrarily primed polymerase chain reaction (AP-PCR)]. First, using whole-genome sequencing, this study confirmed an exemplary similarity between the oral and tumoral strains derived from each patient with CRC. Second, we successfully developed a method to genotype this bacterium at the strain level, targeting the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated system, which is hypervariable (defined as -strain genotyping PCR). This method can identify strains in cryopreserved samples and is significantly superior to traditional AP-PCR, which can only be performed on isolates. The new methods have great potential for application in etiological studies of in CRC.
是人类口腔中主要的细菌之一。然而，这种细菌在结直肠癌（CRC）组织中富集，可能参与 CRC 的发展。我们之前的研究表明，使用传统的菌株分型方法[任意引物聚合酶链反应（AP-PCR）]， 在源自口腔的 CRC 组织中存在。首先，通过全基因组测序，本研究证实了来自每位 CRC 患者的口腔和肿瘤菌株之间具有典型的相似性。其次，我们成功开发了一种针对簇状规则间隔短回文重复序列（CRISPR）和 CRISPR 相关系统的菌株水平基因分型方法，该系统具有高度可变性（定义为 - 菌株基因分型 PCR）。这种方法可以识别冷冻保存样本中的 菌株，并且明显优于只能在分离物上进行的传统 AP-PCR。这些新方法在 CRC 中 菌株的病因学研究中具有很大的应用潜力。
