Sinclair M I, Maxwell P C, Lyon B R, Holloway B W
J Bacteriol. 1986 Dec;168(3):1302-8. doi: 10.1128/jb.168.3.1302-1308.1986.
The soil isolate Pseudomonas putida MW1000 can grow on toluene and other hydrocarbons; in this respect it is similar to strains of Pseudomonas which carry the TOL plasmid. By conjugation experiments, the genes conferring these growth abilities have been shown to be located on the bacterial chromosome, linked to vil and catB. A 56-kilobase segment of the bacterial chromosome of MW strains carrying the TOL genes can transpose to the IncP-1 plasmid R18-18. Physical analysis of these TOL R18-18 hybrids has shown that the TOL segment is almost identical to the same region found in the TOL plasmid pWW0.
从土壤中分离出的恶臭假单胞菌MW1000能够在甲苯和其他碳氢化合物上生长；在这方面，它与携带TOL质粒的假单胞菌菌株相似。通过接合实验表明，赋予这些生长能力的基因位于细菌染色体上，与vil和catB相连。携带TOL基因的MW菌株的细菌染色体上的一个56千碱基片段可以转座到IncP - 1质粒R18 - 18上。对这些TOL R18 - 18杂种的物理分析表明，TOL片段与在TOL质粒pWW0中发现的相同区域几乎相同。
