具有对草甘膦高耐受性的新型5-烯醇丙酮莽草酸-3-磷酸合酶（AroA），由从中国一个极端污染环境中分离出的恶臭假单胞菌4G-1的一个基因编码。
Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China.
作者信息
Sun Yi-Cheng, Chen Yan-Cheng, Tian Zhe-Xian, Li Feng-Mei, Wang Xin-Yue, Zhang Jing, Xiao Zhen-Long, Lin Min, Gilmartin Niamh, Dowling David N, Wang Yi-Ping
机构信息
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100081, People's Republic of China.
出版信息
Appl Environ Microbiol. 2005 Aug;71(8):4771-6. doi: 10.1128/AEM.71.8.4771-4776.2005.
Abstract
Glyphosate has been used globally as a safe herbicide for weed control. It inhibits 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (AroA), which is a key enzyme in the aromatic amino acid biosynthetic pathway in microorganisms and plants. A Pseudomonas putida strain, 4G-1, was isolated from a soil heavily contaminated by glyphosate in China. Its AroA-encoding gene (aroA) has been cloned, sequenced, and expressed in Escherichia coli. Phylogenetic analysis revealed that this AroA belongs neither to class I nor to class II AroA enzymes. When compared with E. coli AroA, 4G-1 AroA shows similar values for K(m)[PEP], K(m)[S3P], and specific enzyme activity. Moreover, 4G-1 AroA exhibits high tolerance to glyphosate, which indicates a protein with a high potential for structural and functional studies of AroA in general and its potential usage for the generation of transgenic crops resistant to the herbicide.
摘要
草甘膦在全球范围内被用作一种安全的除草剂来控制杂草。它抑制5-烯醇丙酮酰莽草酸-3-磷酸（EPSP）合酶（AroA），这是微生物和植物芳香族氨基酸生物合成途径中的一种关键酶。从中国受草甘膦严重污染的土壤中分离出了恶臭假单胞菌菌株4G-1。其编码AroA的基因（aroA）已被克隆、测序并在大肠杆菌中表达。系统发育分析表明，这种AroA既不属于I类也不属于II类AroA酶。与大肠杆菌AroA相比，4G-1 AroA在K(m)[PEP]、K(m)[S3P]和比酶活性方面表现出相似的值。此外，4G-1 AroA对草甘膦表现出高耐受性，这表明该蛋白在一般情况下对AroA进行结构和功能研究具有很高的潜力，以及其在培育抗除草剂转基因作物方面的潜在用途。
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Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China.具有对草甘膦高耐受性的新型5-烯醇丙酮莽草酸-3-磷酸合酶（AroA），由从中国一个极端污染环境中分离出的恶臭假单胞菌4G-1的一个基因编码。
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