恶臭假单胞菌mt-2的TOL质粒切割图谱。
A cleavage map of the TOL plasmid of Pseudomonas putida mt-2.
作者信息
Downing R, Broda P
出版信息
Mol Gen Genet. 1979;177(1):189-91. doi: 10.1007/BF00267270.
PMID:231727
Abstract
A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII digest products and (iii) analysis of a number of deletion mutants.
摘要
已确定了针对限制性内切酶HindIII和XhoI的TOL质粒pWWO的切割图谱。采用了多种技术，包括：（i）用第二种酶消化纯化的切割产物；（ii）将纯化的XhoI片段与HindIII消化产物的Southern印迹杂交，以及（iii）分析多个缺失突变体。
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