Sanada T, Ito Y, Sisido M, Imanishi Y
J Biomed Mater Res. 1986 Oct;20(8):1179-95. doi: 10.1002/jbm.820200809.
Polyaminoetherurethaneureas bearing tertiary amino groups in the main chain (M-PAEUU) were synthesized, quaternized (Q-M-PAEUU) and heparinized (H-M-PAEUU). With increasing portions of diisocyanate and with decreasing portions of polyaminoether in the feed, M-PAEUU containing more hydrogen-bonded urea carbonyl groups was prepared. With increasing hydrogen-bonding character of M-PAEUU, the adsorbed bovine serum albumin (BSA) was more denatured. By quaternization of M-PAEUU, the protein adsorption increased, but the denaturation of adsorbed proteins was suppressed. With increasing ratio of hydrogen-bonded urea carbonyl groups in Q-M-PAEUU, the adsorptions of BSA, bovine serum gamma-globulin (B gamma G), and bovine plasma fibrinogen (BPF) were decreased, but the degree of denaturation of adsorbed proteins was increased. In the adsorption to H-M-PAEUU, both the amount and the degree of denaturation of adsorbed proteins were strongly decreased. The dynamic adsorption experiments of plasma proteins showed the behaviors which are similar to the equilibrium adsorption experiments. The decrease of hydrogen-bonded urea linkages and the increase of hydrophilicity by quaternization and heparinization of the polymer surface may be favorable for building up a hydration layer on the surface, thus suppressing the denaturation of plasma proteins which may trigger blood clotting and thrombus formation.
合成了主链带有叔氨基的聚氨基醚聚氨酯脲（M-PAEUU），对其进行季铵化（Q-M-PAEUU）和肝素化（H-M-PAEUU）。随着进料中异氰酸酯比例增加和聚氨基醚比例降低，制备出含有更多氢键结合脲羰基的M-PAEUU。随着M-PAEUU氢键结合特性增强，吸附的牛血清白蛋白（BSA）变性程度更高。通过对M-PAEUU进行季铵化，蛋白质吸附量增加，但吸附蛋白质的变性受到抑制。随着Q-M-PAEUU中氢键结合脲羰基比例增加，BSA、牛血清γ-球蛋白（BγG）和牛血浆纤维蛋白原（BPF）的吸附量降低，但吸附蛋白质的变性程度增加。在对H-M-PAEUU的吸附中，吸附蛋白质的量和变性程度均大幅降低。血浆蛋白的动态吸附实验显示出与平衡吸附实验相似的行为。聚合物表面通过季铵化和肝素化使氢键结合脲键减少以及亲水性增加，可能有利于在表面形成水化层，从而抑制可能引发血液凝固和血栓形成的血浆蛋白变性。
