Kim Hye Won, Kim Na Kyung, Phillips Alex P R, Parker David A, Liu Ping, Whitaker Rachel J, Rao Christopher V, Mackie Roderick I
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Champaign, IL, United States.
Materials Research Laboratory, Energy and Biosciences Institute, University of Illinois at Urbana-Champaign, Champaign, IL, United States.
Front Microbiol. 2023 Sep 26;14:1232587. doi: 10.3389/fmicb.2023.1232587. eCollection 2023.
has been isolated from extreme environments such as hot springs, volcanoes, as well as pasteurized acidic beverages, because it can tolerate extreme temperatures and acidity. In our previous study, was isolated during the enrichment of methane oxidizing bacteria from Yellowstone Hot Spring samples.
Physiological characterization and genomic exploration of two new isolates, AL01A and AL05G, are the main focus of this study to identify their potential relationships with a thermoacidophilic methanotroph () isolated from the same hot spring sediments.
In the present study, both isolates showed optimal growth at pH 3.5 and 55°C, and contain ω-alicyclic fatty acids as a major lipid ( 60%) in the bacterial membrane. Genomic analysis of these strains revealed specific genes and pathways that the methanotroph genome does not have in the intermediary carbon metabolism pathway such as (phosphoserine aminotransferase), (phosphosulfolactate synthase), and DAK (glycerone kinase). Both strains were also found to contain transporter systems for extracellular sulfate (ABC transporter), suggesting that they could play an important role in sulfur metabolism in this extreme environment. Genomic analysis of vitamin metabolism revealed and are able to complement each other's nutritional deficiencies, resulting in a mutually beneficial relationship, especially in vitamin B(thiamin), B (niacin), and B (biotin) metabolism. These findings provide insights into the role of isolates in geothermal environments and their unique metabolic adaptations to these environments.
已从温泉、火山等极端环境以及巴氏杀菌酸性饮料中分离出来，因为它能耐受极端温度和酸度。在我们之前的研究中，是从黄石温泉样本中富集甲烷氧化菌的过程中分离出来的。
本研究的主要重点是对两个新的分离株AL01A和AL05G进行生理特性分析和基因组探索，以确定它们与从同一温泉沉积物中分离出的嗜热嗜酸甲烷营养菌（）的潜在关系。
在本研究中，两个分离株在pH 3.5和55°C下均表现出最佳生长，并且在细菌细胞膜中含有ω-脂环族脂肪酸作为主要脂质（60%）。对这些菌株的基因组分析揭示了甲烷营养菌基因组在中间碳代谢途径中没有的特定基因和途径，如（磷酸丝氨酸转氨酶）、（磷酸磺基乳酸合酶）和DAK（甘油酮激酶）。还发现这两个菌株都含有细胞外硫酸盐转运系统（ABC转运蛋白），表明它们可能在这种极端环境中的硫代谢中发挥重要作用。维生素代谢的基因组分析表明，和能够互补彼此的营养缺陷，从而形成互利关系，特别是在维生素B（硫胺素）、B（烟酸）和B（生物素）代谢方面。这些发现为分离株在地热环境中的作用及其对这些环境的独特代谢适应性提供了见解。
