Xu Benjin, Hou Zhuru, Liu Ling, Yan Rongrong, Zhang Jinjing, Wei Jianhong, Du Miao, Xuan Yan, Fan Lei, Li Zhuoxi
Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang, People's Republic of China.
Key Laboratory of Lvliang for Clinical Molecular Diagnostics, Fenyang, People's Republic of China.
Infect Drug Resist. 2023 Oct 6;16:6567-6586. doi: 10.2147/IDR.S411125. eCollection 2023.
This paper explores the drug resistance, genome and proteome expression characteristics of from a food poisoning event.
A multidrug-resistant Enteritidis strain, labeled as 27A, was isolated and identified from a food poisoning patient. Antimicrobial susceptibility testing determined the resistance of 27A strain to 14 antibiotics. Then, WGS analysis and comparative genomics analysis were performed on 27A, and the functional annotation of resistance genes, virulence genes were performed based on VFDB, ARDB, COG, CARD, GO, KEGG, and CAZY databases. Meanwhile, based on iTRAQ technology, quantitative proteomic analysis was conducted on 27A to analyze the functions and interactions of differentially expressed proteins related to bacterial resistance and pathogenicity.
Strain 27A belonged to ST11 . Enteritidis and was resistant to levofloxacin, ciprofloxacin, ampicillin, piperacillin, and ampicillin/sulbactam. There were 33 drug resistance genes, 384 virulence genes and 2 plasmid replicon, IncFIB(S) and IncFII(S), annotated by WGS. Proteomic analysis revealed significant changes in virulence and drug proteins, which were mainly involved in bacterial pathogenicity and metabolic processes. PPI prediction showed the relationship between virulence proteins and T3SS proteins, and PagN cooperated with proteins related to T3SS to jointly mediate the invasion of 27A strain on the human body. Phylogenetic analysis indicated that . Enteritidis has potential transmission in humans, food, and animals.
This study comprehensively analyzed the drug resistance and virulence phenotypes of . Enteritidis 27A using genomic and proteomic approaches. These helps reveal the drug resistance and virulence mechanisms of . Enteritidis, and provides important information for the source tracing and the prevention of related diseases, which lays a foundation for research on food safety, public health monitoring, and the drug resistance and pathogenicity of . Enteritidis.
本文探讨一起食物中毒事件中肠炎沙门氏菌的耐药性、基因组和蛋白质组表达特征。
从一名食物中毒患者中分离并鉴定出一株多重耐药肠炎沙门氏菌，标记为27A。药敏试验确定了27A菌株对14种抗生素的耐药性。然后，对27A进行全基因组测序（WGS）分析和比较基因组学分析，并基于VFDB、ARDB、COG、CARD、GO、KEGG和CAZY数据库对耐药基因、毒力基因进行功能注释。同时，基于iTRAQ技术对27A进行定量蛋白质组分析，以分析与细菌耐药性和致病性相关的差异表达蛋白的功能及相互作用。
27A菌株属于ST11型肠炎沙门氏菌，对左氧氟沙星、环丙沙星、氨苄西林、哌拉西林和氨苄西林/舒巴坦耐药。通过WGS注释出33个耐药基因、384个毒力基因以及2个质粒复制子IncFIB(S)和IncFII(S)。蛋白质组分析显示毒力和药物相关蛋白有显著变化，主要涉及细菌致病性和代谢过程。蛋白质-蛋白质相互作用（PPI）预测显示了毒力蛋白与三型分泌系统（T3SS）蛋白之间的关系，PagN与T3SS相关蛋白协同作用，共同介导27A菌株对人体的侵袭。系统发育分析表明肠炎沙门氏菌在人、食物和动物之间具有潜在传播性。
本研究采用基因组学和蛋白质组学方法全面分析了肠炎沙门氏菌27A的耐药性和毒力表型。这有助于揭示肠炎沙门氏菌的耐药性和毒力机制，为溯源及相关疾病的预防提供重要信息，为食品安全、公共卫生监测以及肠炎沙门氏菌耐药性和致病性研究奠定基础。
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