School of Pharmacy, Guizhou Medical University, Guiyang 550004, China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guiyang 550004, China; State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China.
School of Pharmacy, Guizhou Medical University, Guiyang 550004, China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guiyang 550004, China.
Ecotoxicol Environ Saf. 2023 Nov 1;266:115560. doi: 10.1016/j.ecoenv.2023.115560. Epub 2023 Oct 10.
The liver is a well-known organ contributing to digestion, hemostasis and detoxification, while liver injury is a world-widely distributed health problem with limited treatment choices. We detected the protective effect of Abrus cantoniensis Hance (ACH) on Carbon tetrachloride-induced (CCl) liver injury in mice. Fifty ICR (Institute of Cancer Research) animals were grouped into five groups of control (a), CCl (d), ACH (25 mg/kg) treated group (c), ACH (50 mg/kg) treated group (b), and ACH (100 mg/kg) treated group (e). Mice in groups d, c, b, and e were given CCl every four days, and treated animals received daily ACH supplementation. The results showed that the daily body weights in CCl-induced animals were slightly lower; however, the weight of ACH-treated mice increased, particularly in the higher dose group. Treatment with CCl led to increased liver weight and liver indices in mice, whereas supplementation with ACH reduced both liver weights and liver indices in animals. Histo-pathological analysis indicated that CCl led to inflammatory cell infiltration and hepatocellular degeneration, with collagenous fibers proliferation in ICR animals. In contrast, supplementation with ACH prominently decreased inflammatory cells and degeneration of hepatocytes and inhibited collagen fiber hyperplasia. Furthermore, the levels or concentrations of AST (p < 0.0001), ALT (p < 0.0001), MDA (p < 0.0001), IL-1β (p < 0.01), TNF-α (p < 0.01) and IL-6 (p < 0.01) were significantly higher in CCl induced ICR animals in group d. However, mice treated with ACH showed lower levels or concentrations of those indices in dose dependent manner. The levels of GSH-px (p < 0.0001), CAT (p < 0.0001) and SOD (p < 0.0001) were significantly reduced in CCl group; however, all these three enzymes exhibited significant (p < 0.05) increase in animals supplemented with ACH in dose dependent manner. The microbiome sequencing generated 1,168,327 filtered reads in the mice samples. A notable difference was observed in the composition of 6 phyla and 37 genera among the five ICR animal groups. Supplementation with ACH increased the abundance of beneficial genera of Coprococcus, Blautia and Clostridium, while concurrently decreased the presence of pathogenic genera of Mycoplasma and Helicobacter. In conclusion, we revealed that Abrus cantoniensis Hance has the potential to relieve liver damage induced by CCl, through the reduction of inflammation, enhancement of antioxidant capacity, and regulation of intestinal microbiota.
肝脏是一个众所周知的器官，有助于消化、止血和解毒，而肝损伤是一个全球性分布的健康问题，治疗选择有限。我们检测了鸡骨草（Abrus cantoniensis Hance，ACH）对四氯化碳（CCl）诱导的肝损伤的保护作用。将 50 只 ICR（癌症研究所）动物分为五组：对照组（a）、CCl 组（d）、ACH（25mg/kg）治疗组（c）、ACH（50mg/kg）治疗组（b）和 ACH（100mg/kg）治疗组（e）。d、c、b 和 e 组的小鼠每四天给予 CCl，并用 ACH 进行每日补充治疗。结果表明，CCl 诱导的动物的每日体重略有下降，而 ACH 治疗的小鼠体重增加，尤其是在高剂量组。CCl 处理导致小鼠的肝重和肝指数增加，而 ACH 补充降低了动物的肝重和肝指数。组织病理学分析表明，CCl 导致 ICR 动物的炎症细胞浸润和肝细胞变性，胶原纤维增生。相比之下，ACH 补充显著减少了炎症细胞和肝细胞变性，并抑制了胶原纤维增生。此外，AST（p<0.0001）、ALT（p<0.0001）、MDA（p<0.0001）、IL-1β（p<0.01）、TNF-α（p<0.01）和 IL-6（p<0.01）的水平或浓度在 CCl 诱导的 d 组 ICR 动物中显著升高。然而，ACH 治疗的小鼠表现出剂量依赖性的较低水平或浓度。CCl 组 GSH-px（p<0.0001）、CAT（p<0.0001）和 SOD（p<0.0001）的水平显著降低，而 ACH 补充的动物这些酶均呈剂量依赖性显著增加（p<0.05）。微生物组测序在小鼠样本中产生了 1168327 个过滤读数。五个 ICR 动物组之间，6 个门和 37 个属的组成有明显差异。ACH 补充增加了有益属粪肠球菌、真杆菌和梭菌的丰度，同时降低了致病性属支原体和螺旋杆菌的丰度。综上所述，我们发现鸡骨草通过减轻炎症、增强抗氧化能力和调节肠道微生物群，具有缓解 CCl 诱导的肝损伤的潜力。
