College of Animal Science and Technology, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi Grass Station, Guangxi University, Nanning 530004, Guangxi, China.
Center of Bioinformatics, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Int J Biol Macromol. 2024 Oct;278(Pt 2):134532. doi: 10.1016/j.ijbiomac.2024.134532. Epub 2024 Aug 13.
Abrus cantoniensis Polysaccharides (ACP) exhibit antioxidant activity and immune-regulatory functions. Abrus cantoniensis Hance widely distributed in the Guangdong and Guangxi regions of China. In this study, this research investigated the impact of phosphorylation modification on the biological activity of ACP, aiming to provide theoretical insights for its development. This research modified ACP through phosphorylation and evaluated changes in its in vitro antioxidant capacity, including free radical scavenging and resistance to cellular oxidative damage. Additionally, this research administered both native ACP and phosphorylated ACP (P-ACP) to mice to assess their protective effects against acute ethanol-induced oxidative injury. This research explored whether these effects were mediated through the Keap1-Nrf2 signaling pathway and their influence on gut microbiota. Results revealed that phosphorylation significantly enhanced ACP's antioxidant capacity and protective effects (p < 0.05). P-ACP improved mice resistance to acute oxidative injury, mitigating the adverse effects of 50 % ethanol (p < 0.05). Moreover, both ACP and P-ACP are involved in modulating the expression of the Keap1-Nrf2 signaling pathway and, to some extent, alter the composition of the gut microbiota in mice. In summary, phosphorylation modification effectively enhances ACP's antioxidant capacity and provides better protection against acute oxidative injury in mice.
鸡骨草多糖（ACP）具有抗氧化活性和免疫调节功能。鸡骨草（ Abrus cantoniensis ）广泛分布于中国广东和广西地区。本研究旨在探讨磷酸化修饰对 ACP 生物学活性的影响，为其开发提供理论依据。本研究通过磷酸化修饰 ACP，并评估其体外抗氧化能力的变化，包括自由基清除能力和抵抗细胞氧化损伤的能力。此外，本研究还将天然 ACP 和磷酸化 ACP（P-ACP）给予小鼠，以评估它们对急性乙醇诱导的氧化损伤的保护作用。本研究探讨了这些作用是否通过 Keap1-Nrf2 信号通路介导，以及它们对肠道微生物群的影响。结果表明，磷酸化显著增强了 ACP 的抗氧化能力和保护作用（p<0.05）。P-ACP 提高了小鼠对急性氧化损伤的抵抗力，减轻了 50%乙醇的不良影响（p<0.05）。此外，ACP 和 P-ACP 都参与调节 Keap1-Nrf2 信号通路的表达，并在一定程度上改变了小鼠肠道微生物群的组成。综上所述，磷酸化修饰可有效增强 ACP 的抗氧化能力，并为小鼠急性氧化损伤提供更好的保护。
