College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; MOE JoInt. International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Hubei Three Goreges Polytechnic, Yichang 443199, China.
Ecotoxicol Environ Saf. 2024 Jul 1;279:116495. doi: 10.1016/j.ecoenv.2024.116495. Epub 2024 May 30.
Abrus cantoniensis Hance (ACH) is an ancient Chinese medicine herb known for its therapeutic effects. This study investigated the potential protective effect of ACH against carbon tetrachloride (CCl)-induced liver damage in mice. Fifty (n= 50) ICR mice were grouped into five groups. CCl was intraperitoneally injected into different mice groups: AM (CCl induced), AD (ACH-treated with 25 mg/kg), AZ (ACH-treated with 50 mg/kg), and AG (ACH-treated with100mg/kg) after every three days for a total of 31 days. The control group was denoted as AC. Additionally, groups AD, AZ, and AG received daily doses of ACH via gavage throughout the study period. According to our findings, ACH administration prominently mitigated liver pathological lesions and the increased liver index induced by CCl in mice (p < 0.05). Treatment with ACH resulted in a dose-dependent recovery of GSH-px, SOD, and CAT activities (p < 0.001). Moreover, the levels of TNF-α, MDA, and ALT showed significanlty decreasing trends with various doses of ACH (p < 0.001). Furthermore, 16 S rRNA gene sequencing demonstrated that ACH increased the abundance of beneficial genera of Comoclathris, Aureobasidium, and Kazachstania while decreased the presence of pathogenic genera such as Sporobolomyces and Filobasidium. Additionally, ACH treatment ameliorated the changes in liver metabolism due to CCl and enhanced the beneficial liver metabolites. In conclusion, ACH shows potential in protecting the liver against oxidative stress and inflammation caused by CCl exposure, possibly through its effects on gut microbiota and liver metabolism. Therefore, the use of ACH may offer an effective approach for alleviating CCl-induced liver injury.
鸡骨草（Abrus cantoniensis Hance，ACH）是一种具有治疗作用的传统中药。本研究旨在探讨鸡骨草对四氯化碳（CCl）诱导的小鼠肝损伤的潜在保护作用。将 50 只 ICR 小鼠随机分为 5 组，每组 10 只。除 AC 组外，其余 4 组小鼠均采用腹腔注射的方式给予 CCl 造模，每隔 3 天注射 1 次，共注射 31 次。AM 组给予生理盐水，AD 组、AZ 组和 AG 组分别给予鸡骨草提取物 25、50 和 100mg/kg，AG 组在造模的同时给予鸡骨草提取物灌胃。造模结束后，处死所有小鼠，检测其肝脏病理变化、肝指数、氧化应激相关指标、肠道菌群及肝脏代谢物的变化。结果显示，鸡骨草提取物可显著减轻 CCl 诱导的小鼠肝脏病理损伤和肝指数升高（p<0.05），且呈剂量依赖性；鸡骨草提取物还可提高 GSH-px、SOD 和 CAT 等抗氧化酶的活性（p<0.001），降低 TNF-α、MDA 和 ALT 等炎症因子的水平（p<0.001）。16S rRNA 基因测序结果显示，鸡骨草提取物可增加有益菌（Comoclathris、Aureobasidium 和 Kazachstania）的相对丰度，降低致病菌（Sporobolomyces 和 Filobasidium）的相对丰度，改善 CCl 引起的肠道菌群失调；鸡骨草提取物还可改善 CCl 引起的肝脏代谢紊乱，增强有益代谢物。综上所述，鸡骨草提取物可能通过调节肠道菌群和改善肝脏代谢，减轻 CCl 诱导的氧化应激和炎症反应，对 CCl 诱导的肝损伤具有一定的保护作用。
