• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Preparation of phosphorylcholine-conjugated antigens.

作者信息

Rifai A, Wong S S

出版信息

J Immunol Methods. 1986 Nov 20;94(1-2):25-30. doi: 10.1016/0022-1759(86)90211-5.

DOI:10.1016/0022-1759(86)90211-5
PMID:3782815
Abstract

In response to the need for a phosphorylcholine (PC) hapten that could be attached easily to carrier molecules, synthesis of PC-carrier conjugates was undertaken. An isothiocyanate-PC derivative was prepared by mixing p-aminophenyl-PC with thiophosgene. The isothiocyanate-PC derivative was coupled directly to aminoethylated Ficoll at room temperature in a bicarbonate buffer. Maximum coupling, 31 PC groups per Ficoll molecule, occurred within 16 h. The number of PC per Ficoll was determined spectrophotometrically at 245 nm. This approach was also successively used for conjugating PC to bovine serum albumin and aminoethylated Sepharose.

摘要

相似文献

1
Preparation of phosphorylcholine-conjugated antigens.
J Immunol Methods. 1986 Nov 20;94(1-2):25-30. doi: 10.1016/0022-1759(86)90211-5.
2
Preparation of phosphorylcholine derivatives of bovine serum albumin and their application to the affinity chromatography of C-reactive protein.
Anal Biochem. 1987 Mar;161(2):567-73. doi: 10.1016/0003-2697(87)90490-8.
3
Studies on immune responses to parasite antigens in mice. IV. Inhibition of an anti-DNP antibody response with the antigen, DNP-Ficoll containing phosphorylcholine.
Int Arch Allergy Appl Immunol. 1976;52(1-4):235-40.
4
Photochemical linking of primary aromatic amines to carrier proteins to elicit antibody response against the amine haptens.将伯芳香胺与载体蛋白进行光化学连接，以引发针对胺类半抗原的抗体反应。
J Immunol Methods. 1986 Nov 20;94(1-2):237-46. doi: 10.1016/0022-1759(86)90238-3.
5
Binding of phosphorylcholine by non-immunoglobulin molecules on mouse B cells.小鼠B细胞上非免疫球蛋白分子对磷酸胆碱的结合
J Immunol. 1983 Jul;131(1):365-9.
6
The immune response in NZB mice of different ages to thymus-dependent and thymus-independent phosphorylcholine antigens.不同年龄的新西兰黑鼠对胸腺依赖性和非胸腺依赖性磷酸胆碱抗原的免疫反应。
Immunology. 1978 Jun;34(6):1063-9.
7
Demonstration of phosphorylcholine-specific IgE B cells in CBA/N mice.在CBA/N小鼠中磷酸胆碱特异性IgE B细胞的证明。
J Immunol. 1979 Sep;123(3):1039-43.
8
Phosphorylcholine on isologous red blood cells induces polyclonal but not anti-phosphorylcholine plaque-forming cells in mice.同种红细胞上的磷酸胆碱可诱导小鼠产生多克隆而非抗磷酸胆碱的空斑形成细胞。
Eur J Immunol. 1984 Jul;14(7):595-8. doi: 10.1002/eji.1830140703.
9
Preparation of stable erythrocyte target cells suitable for detection of the antibody response to the haptens azobenzenearsonate, azophenyltrimethylammonium and azophenylphosphorylcholine by plaque-forming cell assay.制备适用于通过空斑形成细胞试验检测对半抗原偶氮苯胂酸、偶氮苯基三甲基铵和偶氮苯磷酰胆碱抗体反应的稳定红细胞靶细胞。
J Immunol Methods. 1979;25(1):89-96. doi: 10.1016/0022-1759(79)90169-8.
10
Analysis of T cell reactivities to phosphorylcholine-conjugated hen egg lysozyme in C57BL/6 mice: hapten-conjugate specificity reflects an altered expression of a major carrier epitope.C57BL/6小鼠中T细胞对磷酸胆碱偶联的鸡卵溶菌酶的反应性分析：半抗原偶联物特异性反映了主要载体表位表达的改变。
Eur J Immunol. 1991 May;21(5):1303-10. doi: 10.1002/eji.1830210531.

引用本文的文献

1
Labeling primary amine groups in peptides and proteins with N-hydroxysuccinimidyl ester modified Fe3O4@SiO2 nanoparticles containing cleavable disulfide-bond linkers.用含可裂解二硫键连接体的 N-羟基琥珀酰亚胺酯修饰的 Fe3O4@SiO2 纳米粒子标记肽和蛋白质中的伯胺基团。
Bioconjug Chem. 2013 Sep 18;24(9):1562-9. doi: 10.1021/bc400165r. Epub 2013 Aug 28.
2
Clearance kinetics, tissue localization and fate of IgA-anti-idiotype complexes.IgA抗独特型复合物的清除动力学、组织定位及转归
Immunology. 1990 Apr;69(4):610-5.