Kishimoto T, Shigemoto S, Watanabe T, Yamamura Y
J Immunol. 1979 Sep;123(3):1039-43.
CBA/N mice, which did not make anti-PC IgM or IgG antibody against PC-conjugated T-dependent or T-independent antigens, produced IgE antibody to PC-determinant when they were immunized with PC-KLH. PC-specificity of IgE antibody produced in CBA/N mice was determined by inhibition of PCA reaction with free PC-hapten or C-polysaccharide or by absorption of reaginic activity in the serum with C-polysaccharide. The presence of T15 idiotype on anti-PC IgE antibody produced in CBA/N x BALB/c F1 males also showed that anti-PC IgE antibody in defective mice was PC-specific. The results suggest that PC-specific B epsilon cells may belong to a subpopulation distinct from PC-specific precursors for IgM and IgG responses.
CBA/N小鼠不能产生针对与PC偶联的T细胞依赖性或T细胞非依赖性抗原的抗PC IgM或IgG抗体，但在用PC-KLH免疫时会产生针对PC决定簇的IgE抗体。通过用游离PC半抗原或C多糖抑制PCA反应，或用C多糖吸收血清中的反应素活性，来确定CBA/N小鼠产生的IgE抗体的PC特异性。在CBA/N×BALB/c F1雄性小鼠中产生的抗PC IgE抗体上存在T15独特型，这也表明缺陷小鼠中的抗PC IgE抗体具有PC特异性。结果表明，PC特异性Bε细胞可能属于一个与IgM和IgG反应的PC特异性前体不同的亚群。
