Argunsah Ali Özgür, Israely Inbal
Laboratory of Neuronal Circuit Assembly, Brain Research Institute (HiFo), University of Zurich, Zurich, Switzerland.
Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Kadir Has University, Istanbul, Türkiye.
Front Cell Neurosci. 2023 Sep 27;17:1253446. doi: 10.3389/fncel.2023.1253446. eCollection 2023.
Dendritic spines are highly dynamic structures whose structural and functional fluctuations depend on multiple factors. Changes in synaptic strength are not limited to synapses directly involved in specific activity patterns. Unstimulated clusters of neighboring spines in and around the site of stimulation can also undergo alterations in strength. Usually, when plasticity is induced at single dendritic spines with glutamate uncaging, neighboring spines do not show any significant structural fluctuations. Here, using two-photon imaging and glutamate uncaging at single dendritic spines of hippocampal pyramidal neurons, we show that structural modifications at unstimulated neighboring spines occur and are a function of the temporal pattern of the plasticity-inducing stimulus. Further, the relative location of the unstimulated neighbors within the local dendritic segment correlates with the extent of heterosynaptic plasticity that is observed. These findings indicate that naturalistic patterns of activity at single spines can shape plasticity at nearby clusters of synapses, and may play a role in priming local inputs for further modifications.
树突棘是高度动态的结构，其结构和功能波动取决于多种因素。突触强度的变化不仅限于直接参与特定活动模式的突触。刺激部位及其周围未受刺激的相邻棘突簇的强度也会发生改变。通常，当用谷氨酸光解笼锁技术在单个树突棘上诱导可塑性时，相邻的棘突不会表现出任何明显的结构波动。在这里，我们利用双光子成像和谷氨酸光解笼锁技术，对海马锥体神经元的单个树突棘进行研究，结果表明，未受刺激的相邻棘突会发生结构改变，且这种改变是可塑性诱导刺激时间模式的函数。此外，局部树突段内未受刺激的相邻棘突的相对位置与观察到的异突触可塑性程度相关。这些发现表明，单个棘突的自然活动模式可以塑造附近突触簇的可塑性，并可能在启动局部输入以进行进一步修饰方面发挥作用。
