Samir Haney, Mandour Ahmed S, Radwan Faten, Ahmed Ahmed Ezzat, Momenah Maha Abdullah, Aldawood Nouf Arkan, Yoshida Tomihiko, Watanabe Gen, El-Sherbiny Hossam R
Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-Cho, Fuchu, Tokyo 183-8509, Japan.
Animals (Basel). 2023 May 29;13(11):1794. doi: 10.3390/ani13111794.
The beneficial effects of melatonin were investigated to mitigate various detrimental effects and toxicity on reproductive performance. The present study aimed, for the first time, to explore the effect of intravenous melatonin injection on testicular artery hemodynamics (TH) and metabolomic changes, reproductive hormones in heat-stressed bucks. Ten bucks were randomly split into two groups (five each): (1) the melatonin group, treated with a single intravenous dose of melatonin solution containing 10 mg melatonin each, and (2) the control group, which was treated with 10 mL of the vehicle without melatonin. Changes in the TH at the level of the supra testicular artery (STA) were assessed by triplex ultrasonography just before (0 h) and at 0.5, 2, 7, 24, and 168 h after melatonin or vehicle administration. Doppler velocity parameters of peak systolic velocity (PSV; cm/s), end-diastolic velocity (EDV; cm/s), and time average maximum velocity (TAMAX; cm/s) were measured. Doppler indices (resistive index; RI and pulsatility index; PI), systole/diastole (S/D) ratio and total arterial blood flow volume (TABFV; ml/minute) were measured. Peripheral concentrations of FSH, LH, inhibin, melatonin, testosterone (T), estradiol (E2), and cortisol were measured just before injection (0 h) and at 0.5, 2, 7, and 24 h and daily up to day 7 post administration in both groups. Results revealed reductions in the RI values and increases in the TABFV in the melatonin group compared to the control one, especially 2 h after administration. Significant increases in concentrations of FSH, T, E2, and melatonin and decreases in cortisol and inhibin in the melatonin group compared to the control one. Plasma metabolomic analysis at 2 h indicated the up-regulation of L-glutamine, L-arginine, sorbitol, D-glucose, ascorbic acid, and ornithine and the down-regulation of D-xylose, D-arabitol, ribitol, and oleic acid in the melatonin versus the control group. In conclusion, acute administration of melatonin (10 mg IV) enhanced testicular artery blood flow and plasma reproductive hormones in the Shiba goat under heat-stress circumstances.
研究了褪黑素减轻对生殖性能的各种有害影响和毒性的有益作用。本研究首次旨在探讨静脉注射褪黑素对热应激公山羊睾丸动脉血流动力学（TH）、代谢组学变化及生殖激素的影响。将10只公山羊随机分为两组（每组5只）：（1）褪黑素组，静脉注射单剂量含10 mg褪黑素的溶液；（2）对照组，注射10 mL不含褪黑素的赋形剂。在注射褪黑素或赋形剂前（0小时）以及注射后0.5、2、7、24和168小时，通过三维超声评估睾丸上动脉（STA）水平的TH变化。测量收缩期峰值流速（PSV；cm/s）、舒张末期流速（EDV；cm/s）和时间平均最大流速（TAMAX；cm/s）的多普勒速度参数。测量多普勒指数（阻力指数；RI和搏动指数；PI）、收缩期/舒张期（S/D）比值和总动脉血流量（TABFV；ml/分钟）。在注射前（0小时）以及注射后0.5、2、7和24小时以及给药后第7天每天测量两组外周血中促卵泡生成素（FSH）、促黄体生成素（LH）、抑制素、褪黑素、睾酮（T）、雌二醇（E2）和皮质醇的浓度。结果显示，与对照组相比，褪黑素组的RI值降低，TABFV增加，尤其是给药后2小时。与对照组相比，褪黑素组FSH、T、E2和褪黑素浓度显著升高，皮质醇和抑制素浓度降低。2小时时的血浆代谢组学分析表明，与对照组相比，褪黑素组中L-谷氨酰胺、L-精氨酸、山梨醇、D-葡萄糖、抗坏血酸和鸟氨酸上调，D-木糖、D-阿拉伯糖醇、核糖醇和油酸下调。总之，在热应激情况下，急性注射褪黑素（10 mg静脉注射）可增强柴山羊的睾丸动脉血流量和血浆生殖激素水平。
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