Departement of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia.
Undergraduate Pharmacy Study Programme, Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia.
J Complement Integr Med. 2023 Oct 16;20(4):740-747. doi: 10.1515/jcim-2022-0416. eCollection 2023 Dec 1.
Vernonia amygdalina (VA) is a plant that consumed as vegetable by Indonesians contained numerous secondary metabolites. VA's pharmacological action, including its antioxidant properties, anticancer, antidiabetic, and hepatoprotective. The purpose of this research is to reveal the activity of . leafs aqueous fraction (VALAF) as a blood pressure-lowering agent in hypertensive model.
Combination of prednisone and NaCl were used as hypertensive inducer. The animals were split into five different groups, normal control group treated with distilled water, treatment VALAF groups with dose of 10; 20 and 40 mg/kg BW respectively, while the last group was treated with captopril at dose of 2.25 mg/kg BW. All animals were given an oral treatment for 15 days. On days 5, 10, and 15, systolic and diastolic blood pressure, heart rate (HR), mean arterial pressure (MAP), and blood flow (BF) were all measured. On days 0 and 15, NO level were assessed. All data were analyzed using two-way ANOVA, and Duncan Multiple Range Test.
The leaf aqueous fraction has blood pressure lowering activity. The blood pressure parameter of the rats treated with VALAF were lower as compared to the normal control group (p<0.05). NO levels in the VALAF group were not significantly higher than in the normal control group (p>0.05). The VALAF 20 give the greatest percentage of decrease in blood pressure, heart rate and blood volume on the 15 day of examination.
These study indicated that leaf aqueous fraction has the potential to be an alternative therapy for managing blood pressure in hypertensive animal models.
山芝麻（VA）是一种被印度尼西亚人作为蔬菜食用的植物，含有许多次生代谢产物。VA 的药理作用包括抗氧化、抗癌、抗糖尿病和保肝作用。本研究的目的是揭示. 叶水提物（VALAF）作为降压剂在高血压模型中的活性。
泼尼松和 NaCl 的组合用于诱导高血压。将动物分为五组，正常对照组给予蒸馏水，VALAF 治疗组分别给予 10、20 和 40mg/kg BW 的剂量，最后一组给予卡托普利 2.25mg/kg BW 的剂量。所有动物均给予口服治疗 15 天。在第 5、10 和 15 天，测量收缩压和舒张压、心率（HR）、平均动脉压（MAP）和血流量（BF）。在第 0 和 15 天，评估 NO 水平。所有数据均采用双因素方差分析和 Duncan 多重范围检验进行分析。
. 叶水提物具有降压作用。与正常对照组相比，VALAF 治疗组的大鼠血压参数较低（p<0.05）。VALAF 组的 NO 水平与正常对照组相比没有显著升高（p>0.05）。VALAF 20 在第 15 天检查时对血压、心率和血流量的降低百分比最大。
这些研究表明，. 叶水提物具有作为高血压动物模型降压治疗替代疗法的潜力。
