Institute of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Shanxi Key Laboratory of Material Strength & Structural Impact, Taiyuan, Shanxi, 030024, China; Research Center for Nanobiomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi, 030024, China.
Research Center for Nanobiomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi, 030024, China.
J Mech Behav Biomed Mater. 2023 Dec;148:106162. doi: 10.1016/j.jmbbm.2023.106162. Epub 2023 Oct 9.
Electrospun fibres have been widely used as skin dressings due to their unique structur. However, due to the lack of intrinsic antimicrobial activity, it is easy for the wound to become infected. Bacterial infection, which leads to chronic inflammation, severely hinders the normal process of skin regeneration. In this study, a polyvinyl alcohol/chitosan (PVA/CS) composite films with chemical sterilization and near-infrared (NIR) photothermal antibacterial activity was fabricated by electrospinning. Graphene oxide (GO), a photosensitiser, was incorporated into the films, and lanthanum chloride (Lacl) as a chemical antibacterial agent was also doped in the electrospun films. The structure, morphology, mechanical properties, wettability, and antimicrobial and photothermal antibacterial activity of the PVA/CS-based fibre films were investigated. The results showed that the addition of Lacl to the PVA/CS/GO nanofibres (PVA/CS/GO-La) improved the hydrophilicity, tensile strength and resistance to elastic deformation of the nanofibres. The PVA/CS/GO-La12.5 mM sample exhibited the best antibacterial performance, showing high inhibition against Staphylococcus aureus (82% antibacterial efficacy) and Escherichia coli (99.7% antibacterial efficacy). Furthermore, the antibacterial efficacy of the films surface was further enhanced after exposure to NIR light (808 nm, 0.01 W) for 20 min. In addition, the nanofibre films showed no cytotoxicity against human skin fibroblasts (HSFs), indicating its potential application in the field of broad-spectrum antibacterial materials.
静电纺丝纤维由于其独特的结构，已被广泛用作皮肤敷料。然而，由于缺乏内在的抗菌活性，伤口很容易感染。细菌感染会导致慢性炎症，严重阻碍皮肤再生的正常过程。在这项研究中，通过静电纺丝制备了具有化学杀菌和近红外（NIR）光热抗菌活性的聚乙烯醇/壳聚糖（PVA/CS）复合膜。将一种光敏剂氧化石墨烯（GO）掺入到膜中，并在电纺纤维膜中掺杂了镧氯化物（Lacl）作为化学抗菌剂。研究了基于 PVA/CS 的纤维膜的结构、形态、力学性能、润湿性、抗菌和光热抗菌活性。结果表明，Lacl 的加入提高了 PVA/CS/GO 纳米纤维（PVA/CS/GO-La）的亲水性、拉伸强度和弹性变形阻力。PVA/CS/GO-La12.5mM 样品表现出最佳的抗菌性能，对金黄色葡萄球菌（82%的抗菌效力）和大肠杆菌（99.7%的抗菌效力）表现出高抑制作用。此外，纳米纤维膜在暴露于近红外光（808nm，0.01W）20min 后，其表面的抗菌效果进一步增强。此外，纳米纤维膜对人皮肤成纤维细胞（HSFs）没有细胞毒性，表明其在广谱抗菌材料领域具有潜在的应用。
