Academy of National Food and Strategic Reserves Administration, Beijing 100037, China.
Academy of National Food and Strategic Reserves Administration, Beijing 100037, China; College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 1):126692. doi: 10.1016/j.ijbiomac.2023.126692. Epub 2023 Sep 4.
This study developed a new "capture and killing" antibacterial approach for efficient elimination of foodborne pathogens. FeO-Chitosan (CS)/polyvinyl alcohol (PVA) nanofibrous films with improved antibacterial and mechanical properties were fabricated by a simple, environmentally friendly, and cost-effective electrospinning technique. The relationship between the physical properties (viscosity, surface tension, and conductivity) and spinnability of CS/PVA as fiber forming matrix was explored. Electrospun FeO-CS/PVA films (0.03-0.12 mm) with smooth and bead-free nanofibrous structures (145-169 nm) were successfully obtained. Compared with the film electrospun from neat CS/PVA, incorporating FeO nanoparticles (NPs) (1.25-5 wt%) in CS/PVA nanofibrous film promoted bacterial attachment and increased the final inactivated efficiency, showing a difference with FeO loading and bacterial strain, which had the highest value against Escherichia coli (E. coli) and Staphyloccus aureus (S. aureus) being 90 % and 66.30 %, respectively. The tensile strength and elongation at break of FeO-CS/PVA films enhanced by 46-192 % and 92-141 %, respectively. Results of the cytotoxicity test indicated that the resulting films had high biocompatibility. These promising findings provide a novel strategy for effective foodborne pathogens elimination, which could apply to sterilizing and food packaging to extend the shelf life of liquid food.
本研究开发了一种新的“捕获和杀灭”抗菌方法，可有效消除食源性致病菌。通过简单、环保且经济高效的静电纺丝技术，制备了具有改善的抗菌和机械性能的 FeO-壳聚糖（CS）/聚乙烯醇（PVA）纳米纤维膜。探讨了 CS/PVA 作为纤维形成基质的物理性质（粘度、表面张力和电导率）与可纺性之间的关系。成功获得了具有光滑、无珠纳米纤维结构（145-169nm）的 FeO-CS/PVA 电纺膜（0.03-0.12mm）。与由纯 CS/PVA 电纺的膜相比，在 CS/PVA 纳米纤维膜中掺入 FeO 纳米颗粒（NPs）（1.25-5wt%）可促进细菌附着并提高最终失活效率，表现出与 FeO 负载和细菌菌株的差异，对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的最高灭活效率分别为 90%和 66.30%。FeO-CS/PVA 膜的拉伸强度和断裂伸长率分别提高了 46-192%和 92-141%。细胞毒性试验结果表明，所得到的膜具有良好的生物相容性。这些有前景的发现为有效消除食源性致病菌提供了一种新策略，可应用于液体食品的杀菌和食品包装，以延长其保质期。
