School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, China.
CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
Biosens Bioelectron. 2023 Dec 15;242:115723. doi: 10.1016/j.bios.2023.115723. Epub 2023 Oct 1.
Significant breakthroughs have been made in digital microfluidic (DMF)-based technologies over the past decades. DMF technology has attracted great interest in bioassays depending on automatic microscale liquid manipulations and complicated multi-step processing. In this review, the recent advances of DMF platforms in the biomedical field were summarized, focusing on the integrated design and applications of the DMF system. Firstly, the electrowetting-on-dielectric principle, fabrication of DMF chips, and commercialization of the DMF system were elaborated. Then, the updated droplets and magnetic beads manipulation strategies with DMF were explored. DMF-based biomedical applications were comprehensively discussed, including automated sample preparation strategies, immunoassays, molecular diagnosis, blood processing/testing, and microbe analysis. Emerging applications such as enzyme activity assessment and DNA storage were also explored. The performance of each bioassay was compared and discussed, providing insight into the novel design and applications of the DMF technology. Finally, the advantages, challenges, and future trends of DMF systems were systematically summarized, demonstrating new perspectives on the extensive applications of DMF in basic research and commercialization.
在过去的几十年中，数字微流控（DMF）技术取得了重大突破。DMF 技术因其自动微尺度液体操作和复杂的多步处理而在生物测定中引起了极大的兴趣。在这篇综述中，总结了 DMF 平台在生物医学领域的最新进展，重点介绍了 DMF 系统的集成设计和应用。首先，阐述了介电润湿原理、DMF 芯片的制造以及 DMF 系统的商业化。然后，探讨了具有 DMF 的更新的液滴和磁珠操纵策略。全面讨论了基于 DMF 的生物医学应用，包括自动化样品制备策略、免疫分析、分子诊断、血液处理/检测和微生物分析。还探索了新兴应用，如酶活性评估和 DNA 存储。比较和讨论了每种生物测定的性能，为 DMF 技术的新型设计和应用提供了见解。最后，系统总结了 DMF 系统的优点、挑战和未来趋势，展示了 DMF 在基础研究和商业化中的广泛应用的新视角。
