Key Laboratory of Integrated Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi, 83009, China; Department of Botany, Faculty of Sciences, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, 46300, Pakistan.
Key Laboratory of Integrated Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi, 83009, China; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Plant Physiol Biochem. 2023 Oct;203:108067. doi: 10.1016/j.plaphy.2023.108067. Epub 2023 Oct 5.
Agronomic crops can benefit from the application of nanoscale materials in order to control phytopathogens and improve plant growth. Bipolaris sorokiniana, a soil- and seed-borne fungus, causes severe yield losses in wheat. In order to determine the physio-chemical changes in wheat under biotic stress of B. sorokiniana, the current study aimed to synthesis silver nanoparticles (AgNPs) using Allium sativum bulb extract. Herein, we applied the silver nanoparticles (AgNPs) as a foliar spray on two wheat varieties (Pakistan-2013, and NARC-2011) at the concentrations of 10, 20, 30, and 40 mg/L to suppress B. sorokiniana. Among all the applied concentrations of AgNPs, the 40 mg/L concentration demonstrated the most effective outcome in reduction of the intensity of spot blotch and improved the morphological, physiological, biochemical parameters, as well as antioxidant activity in wheat plant. Foliar application of AgNPs at 40 mg/L Pakistan-2013 and NARC-2011 wheat varieties significantly increased chlorophyll a 84.8% and 53.4%, chlorophyll b 28.9% and 84.3%, total chlorophyll content 294.3% and 241.2%, membrane stability index 7.5% and 6.1%, relative water contents 25.4% and 10.5%, proline content 320.5% and 609.9%, and soluble sugar content 120% and 259.4%, respectively, compared to control and diseased plant. This is the first study provides important insights into the role of phyto-mediated AgNPs in increasing resistant of wheat infected with B. sorokiniana. These findings offers valuable new insights that may be useful for reducing disease incidence in wheat fields.
为了控制植物病原菌并促进植物生长，农业作物可以受益于纳米材料的应用。旋孢腔菌是一种土传和种子传播的真菌，可导致小麦严重减产。为了确定小麦在旋孢腔菌生物胁迫下的生理化学变化，本研究旨在利用大蒜鳞茎提取物合成银纳米粒子（AgNPs）。在此，我们将银纳米粒子（AgNPs）作为叶面喷雾应用于两种小麦品种（巴基斯坦-2013 和 NARC-2011），浓度分别为 10、20、30 和 40mg/L，以抑制旋孢腔菌。在所有应用的 AgNPs 浓度中，40mg/L 浓度在降低斑点病强度和改善小麦植株的形态、生理、生化参数以及抗氧化活性方面表现出最有效的效果。在 40mg/L 的 AgNPs 叶面喷施下，巴基斯坦-2013 和 NARC-2011 小麦品种的叶绿素 a 分别增加了 84.8%和 53.4%，叶绿素 b 分别增加了 28.9%和 84.3%，总叶绿素含量分别增加了 294.3%和 241.2%，膜稳定性指数分别增加了 7.5%和 6.1%，相对含水量分别增加了 25.4%和 10.5%，脯氨酸含量分别增加了 320.5%和 609.9%，可溶性糖含量分别增加了 120%和 259.4%，与对照和病株相比。这是首次研究提供了有关植物介导的 AgNPs 增加感染旋孢腔菌的小麦抗性的重要见解。这些发现提供了有价值的新见解，可能有助于减少小麦田的病害发生。
