Technology & Innovation Centre, Institute of Photonics, University of Strathclyde, 99 George Street, Glasgow G1 1RD, UK.
Int J Mol Sci. 2023 Sep 22;24(19):14416. doi: 10.3390/ijms241914416.
The surface functionalisation of self-assembled colloidal quantum dot supraparticle lasers with a thrombin binding aptamer (TBA-15) has been demonstrated. The self-assembly of CdSSe/ZnS alloyed core/shell microsphere-shape CQD supraparticles emitting at 630 nm was carried out using an oil-in-water emulsion technique, yielding microspheres with an oleic acid surface and an average diameter of 7.3 ± 5.3 µm. Surface modification of the microspheres was achieved through a ligand exchange with mercaptopropionic acid and the subsequent attachment of TBA-15 using EDC/NHS coupling, confirmed by zeta potential and Fourier transform IR spectroscopy. Lasing functionality between 627 nm and 635 nm was retained post-functionalisation, with oleic acid- and TBA-coated microspheres exhibiting laser oscillation with thresholds as low as 4.10 ± 0.37 mJ·cm and 7.23 ± 0.78 mJ·cm, respectively.
已证明可以用凝血酶结合适体（TBA-15）对自组装胶体量子点超粒子激光器进行表面功能化。使用油包水乳液技术进行发射波长为 630nm 的 CdSSe/ZnS 合金核/壳微球形状 CQD 超粒子的自组装，得到具有油酸表面和平均直径为 7.3±5.3µm 的微球。通过巯基丙酸的配体交换和随后使用 EDC/NHS 偶联连接 TBA-15 实现了微球的表面改性，这通过 zeta 电位和傅里叶变换红外光谱得到了证实。功能化后保留了 627nm 到 635nm 之间的激光功能，油酸和 TBA 涂层的微球表现出的激光振荡阈值低至 4.10±0.37mJ·cm 和 7.23±0.78mJ·cm，分别。
