Zhu Liangliang, Hao Hongshun, Ding Chao, Gan Hanwei, Jiang Shuting, Zhang Gongliang, Bi Jingran, Yan Shuang, Hou Hongman
Department of Inorganic Nonmetallic Materials Engineering, Dalian Polytechnic University, Dalian 116034, China.
Liaoning Key Lab for Aquatic Processing Quality and Safety, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Foods. 2021 Nov 23;10(12):2896. doi: 10.3390/foods10122896.
To achieve the rapid detection of , this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO) was used as a photosensitive material, was modified with gold nanoparticles to immobilize complementary DNA, and amplified the signal by means of the sensitization effect of CdTe quantum dots and the shearing effect of Exonuclease I (Exo I) to achieve high-sensitivity detection. This strategy had a detection limit of 45 CFU/mL in the concentration range of 1.3 × 10-1.3 × 10 CFU/mL. The construction strategy provides a new way to detect .
为实现对[具体检测对象未明确]的快速检测，本研究使用适体进行初步识别，并构建了一种基于核酸外切酶辅助扩增的光电化学适体传感器。三氧化钨（WO）用作光敏材料，用金纳米粒子进行修饰以固定互补DNA，并借助碲化镉量子点的敏化作用和核酸外切酶I（Exo I）的剪切作用来放大信号，从而实现高灵敏度检测。该策略在1.3×10 - 1.3×10 CFU/mL的浓度范围内检测限为45 CFU/mL。该构建策略为检测[具体检测对象未明确]提供了一种新方法。
