Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
Institute for Molecular Biosciences, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
Int J Mol Sci. 2023 Sep 28;24(19):14701. doi: 10.3390/ijms241914701.
DNA repair in mammalian cells involves the coordinated action of a range of complex cellular repair machinery. Our understanding of these DNA repair processes has advanced to the extent that they can be leveraged to improve the efficacy and precision of Cas9-assisted genome editing tools. Here, we review how the fusion of CRISPR-Cas9 to functional domains of proteins that directly or indirectly impact the DNA repair process can enhance genome editing. Such studies have allowed the development of diverse technologies that promote efficient gene knock-in for safer genome engineering practices.
哺乳动物细胞中的 DNA 修复涉及一系列复杂的细胞修复机制的协调作用。我们对这些 DNA 修复过程的理解已经发展到可以利用它们来提高 Cas9 辅助基因组编辑工具的效率和精度。在这里，我们回顾了 CRISPR-Cas9 与直接或间接影响 DNA 修复过程的蛋白质功能域融合如何增强基因组编辑。这些研究允许开发促进高效基因敲入的各种技术，以实现更安全的基因组工程实践。
