Moller P C, Chang J P, Partridge L R
J Environ Pathol Toxicol Oncol. 1986 May-Aug;6(5-6):127-42.
The effect of ethylene thiourea (ETU) upon liver cells was evaluated in male Sprague-Dawley rats. ETU was administered ad libitum in drinking water at concentrations of 1, 5, 50, and 500 ppm for time intervals of 1, 2, and 5 days, 1 and 2 weeks, 1, 2, 4, and 8 months. Two additional groups of control animals received ETU-free drinking water or a diet supplemented with 0.06% 3-MeDAB. Electron microscopic evaluation of tissue samples could detect no changes in liver cell morphology of rats receiving 1, 5, or 50 ppm ETU for up to 8 months. By contrast, rats receiving 500 ppm ETU exhibited alterations in hepatic cell morphology after 4 months of exposure. These alterations included a dramatic increase in the amount of smooth endoplasmic reticulum (SER) with a concomitant reduction in rough endoplasmic reticulum (RER), and a relocation of microbodies and mitochondria to the periphery of the SER. No alterations were seen at the shorter time intervals. These changes probably represent a response to the sustained ingestion of high concentrations of highly toxic materials and most likely do not represent a specific response to ETU. No tumors were detected in any of the samples examined or in controls receiving ETU-free drinking water. Animals receiving 3-MeDAB in their diet all developed hepatic tumors within 4 months.
在雄性斯普拉格-道利大鼠中评估了乙烯硫脲（ETU）对肝细胞的影响。ETU以1、5、50和500 ppm的浓度自由添加到饮用水中，给药时间间隔为1、2和5天，1和2周，1、2、4和8个月。另外两组对照动物饮用不含ETU的饮用水或补充有0.06% 3-甲基二乙氨基偶氮苯（3-MeDAB）的饲料。对组织样本的电子显微镜评估未发现接受1、5或50 ppm ETU长达8个月的大鼠肝细胞形态有变化。相比之下，接受500 ppm ETU的大鼠在接触4个月后肝细胞形态出现改变。这些改变包括滑面内质网（SER）数量急剧增加，同时粗面内质网（RER）减少，以及微体和线粒体重新定位到SER周边。在较短时间间隔内未观察到改变。这些变化可能代表对持续摄入高浓度剧毒物质的反应，很可能不代表对ETU的特异性反应。在所检查的任何样本或饮用不含ETU饮用水的对照中均未检测到肿瘤。饮食中接受3-MeDAB的动物在4个月内均发生了肝肿瘤。
