Sahin Demet, Deger Ceren, Oglakci Burcu, Demirkol Metehan, Kucukyildirim Bedri Onur, Gursel Mehtikar, Eliguzeloglu Dalkilic Evrim
Department of Periodontology, Faculty of Dentistry, Bezmialem Vakif University, 34093 Istanbul, Turkey.
Department of Restorative Dentistry, Faculty of Dentistry, Bezmialem Vakif University, 34093 Istanbul, Turkey.
Materials (Basel). 2023 Sep 30;16(19):6522. doi: 10.3390/ma16196522.
This study evaluates the effects of a novel nanohydroxyapatite gel and Er: YAG laser on the surface roughness, surface morphology, and elemental content after dentin hypersensitivity treatments.
Dentin discs (2 × 3 × 3 mm) were prepared from 75 human molars. Out of 75 human molars, 50 were used to evaluate surface roughness and randomly divided into five groups: Group ID (intact dentin), Group DD (demineralized dentin), Group BF (fluoride varnish/Bifluorid 10), Group Lsr (Er: YAG laser-50 mJ, 0.50 W, 10 Hz), and Group NHA (nanohydroxyapatite-containing gel). Dentin hypersensitivity was stimulated by 35% phosphoric acid for 1 min (except Group ID). The surface roughness (Ra, μm) was measured via contact profilometry (n = 10). Out of the 75 sound human molars, 25 were used to evaluate the surface morphology and elemental content using scanning electron microscopy and energy-dispersive X-ray spectroscopy (n = 5). The data were statistically analyzed using Welsch ANOVA, Games-Howell, Kruskal-Wallis, and Dunn tests ( < 0.05).
Group Lsr showed significantly lower surface roughness than Group NHA and Group BF ( < 0.05). The SEM analysis indicated that most of the dentinal tubules were obliterated for Group NHA. Precipitant plugs with partially occluded dentinal tubules were observed for Group BF, while partially or completely occluded tubules with a melting appearance were detected for Group Lsr. The EDS analysis revealed that Group NHA and Group Lsr presented similar calcium and phosphorus amounts to Group ID. All dentin hypersensitivity treatment methods could provide promising results in terms of tubular occlusion efficiency. However, laser treatment resulted in smoother surfaces, which could help prevent dental plaque accumulation.
本研究评估一种新型纳米羟基磷灰石凝胶和铒:钇铝石榴石激光对牙本质过敏症治疗后表面粗糙度、表面形态和元素含量的影响。
从75颗人类磨牙制备牙本质盘(2×3×3毫米)。在75颗人类磨牙中,50颗用于评估表面粗糙度,并随机分为五组:ID组(完整牙本质)、DD组(脱矿牙本质)、BF组(氟化物清漆/双氟化物10)、Lsr组(铒:钇铝石榴石激光-50毫焦、0.50瓦、10赫兹)和NHA组(含纳米羟基磷灰石的凝胶)。除ID组外,用35%磷酸刺激牙本质过敏1分钟。通过接触轮廓仪测量表面粗糙度(Ra,微米)(n = 10)。在75颗健康人类磨牙中,25颗用于使用扫描电子显微镜和能量色散X射线光谱法评估表面形态和元素含量(n = 5)。使用韦尔奇方差分析、盖姆斯-豪厄尔检验、克鲁斯卡尔-沃利斯检验和邓恩检验对数据进行统计学分析(<0.05)。
Lsr组的表面粗糙度明显低于NHA组和BF组(<0.05)。扫描电子显微镜分析表明,NHA组的大多数牙本质小管被封闭。BF组观察到有部分封闭牙本质小管的沉淀栓,而Lsr组检测到有部分或完全封闭且呈熔化外观的小管。能谱分析显示,NHA组和Lsr组的钙和磷含量与ID组相似。所有牙本质过敏症治疗方法在小管封闭效率方面都能提供有前景的结果。然而,激光治疗产生的表面更光滑,这有助于防止牙菌斑积聚。