Ushio Norio, Hasan Md Nazmul, Arif Mohammad, Miura Naoki
United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-0841, Japan.
Joint Graduate School of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Animals (Basel). 2023 Sep 28;13(19):3054. doi: 10.3390/ani13193054.
Hepatocellular carcinomas (HCC) are common tumors, whereas hepatocellular adenomas (HCA) are rare, benign tumors in dogs. The aberrant expression of noncoding RNAs (ncRNAs) plays a pivotal role in HCC tumorigenesis and progression. Among ncRNAs, micro RNAs have been widely researched in human HCC, but much less widely in canine HCC. However, Y RNA-derived fragments have yet to be investigated in canine HCC and HCA. This study targeted canine HCC and HCA patients. We used qRT-PCR to determine Y RNA expression in clinical tissues, plasma, and plasma extracellular vesicles, and two HCC cell lines (95-1044 and AZACH). Y RNA was significantly decreased in tissue, plasma, and plasma extracellular vesicles for canine HCC versus canine HCA and healthy controls. Y RNA was decreased in 95-1044 and AZACH cells versus normal liver tissue and in AZACH versus 95-1044 cells. In plasma samples, Y RNA levels were decreased in HCC versus HCA and Healthy controls and increased in HCA versus Healthy controls. Receiver operating characteristic analysis showed that Y RNA could be a promising biomarker for distinguishing HCC from HCA and healthy controls. Overall, the dysregulated expression of Y RNA can distinguish canine HCC from HCA. However, further research is necessary to elucidate the underlying Y RNA-related molecular mechanisms in hepatocellular neoplastic diseases. To the best of our knowledge, this is the first report on the relative expression of Y RNA in canine HCC and HCA.
肝细胞癌（HCC）是常见肿瘤，而肝细胞腺瘤（HCA）在犬类中是罕见的良性肿瘤。非编码RNA（ncRNA）的异常表达在HCC的肿瘤发生和进展中起关键作用。在ncRNA中，微小RNA在人类HCC中已得到广泛研究，但在犬类HCC中的研究较少。然而，Y RNA衍生片段在犬类HCC和HCA中尚未得到研究。本研究以犬类HCC和HCA患者为对象。我们使用qRT-PCR来确定临床组织、血浆和血浆细胞外囊泡以及两种HCC细胞系（95-1044和AZACH）中Y RNA的表达。与犬类HCA和健康对照相比，犬类HCC的组织、血浆和血浆细胞外囊泡中的Y RNA显著降低。与正常肝组织相比，95-1044和AZACH细胞中的Y RNA降低，与95-1044细胞相比，AZACH细胞中的Y RNA降低。在血浆样本中，与HCA和健康对照相比，HCC中的Y RNA水平降低，与健康对照相比，HCA中的Y RNA水平升高。受试者工作特征分析表明，Y RNA可能是区分HCC与HCA和健康对照的有前景的生物标志物。总体而言，Y RNA的表达失调可区分犬类HCC与HCA。然而，有必要进一步研究以阐明肝细胞肿瘤性疾病中与Y RNA相关的潜在分子机制。据我们所知，这是关于Y RNA在犬类HCC和HCA中相对表达的首次报道。
