Zhang Li, Tang Rui, Wu Yan, Liang Zhenhua, Liu Jingbo, Pi Jinsong, Zhang Hao
Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
Animals (Basel). 2023 Oct 4;13(19):3098. doi: 10.3390/ani13193098.
This study aimed to investigate the effects and mechanisms of retinol and retinoic acid on primary duck intestinal epithelial cells under oxidative stress induced by HO. Different ratios of retinol and retinoic acid were used for treatment. The study evaluated the cell morphology, viability, antioxidative capacity, and barrier function of cells. The expression of genes related to oxidative stress and the intestinal barrier was analyzed. The main findings demonstrated that the treated duck intestinal epithelial cells exhibited increased viability, increased antioxidative capacity, and improved intestinal barrier function compared to the control group. High retinoic acid treatment improved viability and gene expression, while high retinol increased antioxidative indicators and promoted intestinal barrier repair. Transcriptome analysis revealed the effects of treatments on cytokine interactions, retinol metabolism, PPAR signaling, and cell adhesion. In conclusion, this study highlights the potential of retinol and retinoic acid in protecting and improving intestinal cell health under oxidative stress, providing valuable insights for future research.
本研究旨在探讨视黄醇和视黄酸对过氧化氢诱导的氧化应激下原代鸭肠上皮细胞的影响及其机制。采用不同比例的视黄醇和视黄酸进行处理。该研究评估了细胞形态、活力、抗氧化能力和细胞屏障功能。分析了与氧化应激和肠道屏障相关基因的表达。主要研究结果表明，与对照组相比，经处理的鸭肠上皮细胞活力增强、抗氧化能力提高且肠道屏障功能改善。高剂量视黄酸处理可提高细胞活力和基因表达，而高剂量视黄醇则增加抗氧化指标并促进肠道屏障修复。转录组分析揭示了处理对细胞因子相互作用、视黄醇代谢、PPAR信号传导和细胞黏附的影响。总之，本研究突出了视黄醇和视黄酸在氧化应激下保护和改善肠道细胞健康的潜力，为未来研究提供了有价值的见解。
