Lu Guanjie, Wang Zhe, Zhang Baofeng, Zhou Zhichao, Hu Defu, Zhang Dong
School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China.
State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing 100083, China.
Animals (Basel). 2023 Oct 8;13(19):3142. doi: 10.3390/ani13193142.
Currently, researchers use bacterial culture and targeted PCR methods to classify, culture, and identify the pathogens causing abscess diseases. However, this method is limited by factors such as the type of culture medium and culture conditions, making it challenging to screen and proliferate many bacteria effectively. Fortunately, with the development of high-throughput sequencing technology, pathogen identification at the genetic level has become possible. Not only can this approach overcome the limitations of bacterial culture, but it can also accurately identify the types and relative abundance of pathogens. In this study, we used high-throughput sequencing of 16S rRNA to identify the pathogens in purulent fluid samples. Our results not only confirmed the presence of the main pathogen reported by previous researchers, , but also other obligate anaerobes, and as the dominant pathogens causing abscess diseases for the first time. Therefore, our findings suggest that high-throughput sequencing technology has the potential to replace traditional bacterial culture and targeted PCR methods.
目前，研究人员使用细菌培养和靶向PCR方法对引起脓肿疾病的病原体进行分类、培养和鉴定。然而，这种方法受到培养基类型和培养条件等因素的限制，使得有效筛选和增殖许多细菌具有挑战性。幸运的是，随着高通量测序技术的发展，在基因水平上进行病原体鉴定已成为可能。这种方法不仅可以克服细菌培养的局限性，还可以准确鉴定病原体的类型和相对丰度。在本研究中，我们使用16S rRNA高通量测序来鉴定脓性液体样本中的病原体。我们的结果不仅证实了先前研究人员报道的主要病原体的存在，还首次证实了其他专性厌氧菌以及 和 作为引起脓肿疾病的主要病原体。因此，我们的研究结果表明，高通量测序技术有潜力取代传统的细菌培养和靶向PCR方法。
