Heo Ji-Man, Eun Chang-Ho, Kim In-Jung
Department of Biomaterials Engineering, Graduate School, Jeju National University, Jeju 63243, Republic of Korea.
Subtropical Horticulture Research Institute, Jeju National University, Jeju 63243, Republic of Korea.
Plants (Basel). 2023 Sep 22;12(19):3355. doi: 10.3390/plants12193355.
'Miyagawa-wase' mandarin ( Marc. cv. early) is one of the most widely cultivated citrus varieties on Jeju Island in Korea. Mutation breeding is a useful tool for the induction of genetic diversity for the rapid creation of new plant variants. We previously reported the use of gamma irradiation for the development of new citrus varieties. Here, we report a new mutant, , with a unique late fruit ripening phenotype. We investigated the fruit morphological characteristics including weight, vertical/transverse diameter, peel thickness, hardness, and color difference, as well as sugar and acid contents of the compared to wild-type controls. We then used whole genome re-sequencing and functional annotation by gene ontology to identify and characterize single nucleotide polymorphism (SNP) and insertion/deletion (InDel) variants in the , finding a greater abundance of annotated genes containing InDels compared to SNPs. Finally, we used allele-specific PCR to identify molecular markers among the homozygous SNPs detected from the genome sequencing. We report a primer set that effectively distinguishes the from the wild-type control and other citrus varieties. Our findings provide insights into the mechanisms controlling the timing of fruit ripening and tools for the molecular breeding of citrus varieties.
“宫川早生”温州蜜柑（品种：早熟）是韩国济州岛种植最广泛的柑橘品种之一。诱变育种是诱导遗传多样性以快速培育新植物变种的有效手段。我们之前报道了利用伽马射线辐照培育新柑橘品种。在此，我们报道了一个具有独特晚熟果实表型的新突变体。我们研究了该突变体与野生型对照相比的果实形态特征，包括重量、纵横径、果皮厚度、硬度和色差，以及糖和酸含量。然后，我们通过全基因组重测序和基因本体功能注释来识别和表征该突变体中的单核苷酸多态性（SNP）和插入/缺失（InDel）变异，发现与SNP相比，含有InDel的注释基因更为丰富。最后，我们利用等位基因特异性PCR在从该突变体基因组测序中检测到的纯合SNP中鉴定分子标记。我们报道了一组能有效区分该突变体与野生型对照及其他柑橘品种的引物。我们的研究结果为控制果实成熟时间的机制提供了见解，并为柑橘品种的分子育种提供了工具。
