Motalebnejad Masoud, Karimmojeni Hassan, Majidi Mohammad Mahdi, Mastinu Andrea
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Molecular and Translational Medicine, Division of Pharmacology, University of Brescia, 25123 Brescia, Italy.
Plants (Basel). 2023 Sep 22;12(19):3358. doi: 10.3390/plants12193358.
The aim of the current research is to evaluate the allelopathic activity of fifty grass genotypes from different species and to identify phenolic compounds in the genotypes that have the highest allelopathic activity and inhibitory effect on L. (Rocket). Aqueous extract was prepared from the leaves of grass genotypes in different concentrations and its effect on germination and growth of L. was measured. According to the results, the type of genotype and the concentration of the extract significantly decreased the percentage of germination, hypocotyl length, radicle length, and dry weight of L. seedlings. Increasing the concentration of the extract resulted in a decrease in germination and growth of seedlings. The genotypes of (Festulolium) (GR 5009, GR 1692, GR 5004) had the most inhibitory effect on the growth of L. Also, among the genotypes studied, two genotypes (DG-M) and (DG-P) of L. (orchardgrass) species showed the least allelopathic activity. The results of HPLC-MS indicated nine phenolic compounds including caffeic acid, syringic acid, vanillic acid, p-coumaric acid, ferulic acid, apigenin acid, chlorogenic acid, 4-hydroxybenzoic acid, and gallic acid. The phenolic compound most present in the aqueous extract was caffeic acid. However, phenolic compounds derived from genotypes showed the greatest allelopathic action on the growth parameters of L. The aqueous extracts of the genotypes can be considered valid systems of sustainable weed control thanks to the phytocomplex rich in phenols.
当前研究的目的是评估来自不同物种的50种草基因型的化感活性,并鉴定出对独行菜具有最高化感活性和抑制作用的基因型中的酚类化合物。从不同浓度的草基因型叶片中制备水提取物,并测定其对独行菜发芽和生长的影响。结果表明,基因型类型和提取物浓度显著降低了独行菜幼苗的发芽率、下胚轴长度、胚根长度和干重。提取物浓度的增加导致幼苗发芽和生长减少。羊茅属(Festulolium)的基因型(GR 5009、GR 1692、GR 5004)对独行菜的生长抑制作用最大。此外,在所研究的基因型中,鸭茅(orchardgrass)物种的两个基因型(DG-M)和(DG-P)表现出最小的化感活性。高效液相色谱-质谱联用(HPLC-MS)结果表明含有9种酚类化合物,包括咖啡酸、丁香酸、香草酸、对香豆酸、阿魏酸、芹菜素酸、绿原酸、4-羟基苯甲酸和没食子酸。水提取物中含量最多的酚类化合物是咖啡酸。然而,来自这些基因型的酚类化合物对独行菜的生长参数表现出最大的化感作用。由于富含酚类的植物复合物,这些基因型的水提取物可被视为可持续杂草控制的有效体系。