Hussain M Iftikhar, El-Sheikh Mohamed A, Reigosa Manuel J
Department of Plant Biology and Soil Science, Faculty of Biology, University of Vigo, Campus Lagoas-Marcosende, E-36310 Vigo, Spain.
CITACA, Agri-Food Research and Transfer Cluster, Campus da Auga, University of Vigo, 32004 Ourense, Spain.
Plants (Basel). 2020 Sep 18;9(9):1228. doi: 10.3390/plants9091228.
We studied the polyphenol (phenolic compounds and flavonoids) composition and allelopathic effects of R. Br. aerial foliage aqueous extract (0%, 25%, 50%, 75% and 100%) on the seedling growth and plant biomass of the general biotest species, lettuce ). Mean leaf fresh weight, leaf dry weight, root fresh weight and root dry weight were decreased following exposure to aerial foliage, flowers aqueous extract (AFE) and phyllodes aqueous extract (APE) after 6 days. The reduction in plant dry biomass was more than 50% following treatment with AFE. The decrease in mean root length was approximately 37.7% and 29.20% following treatment with flowers extract (AFE) at 75% and 100% concentration, respectively. Root dry weight of was reduced by both flowers and phyllodes extract. The reduction of protein contents in lettuce leaves following foliage extract proved that both AFE and APE exhibit polyphenols that causes the toxicity which led to decrease in leaf protein contents. High-Performance Liquid Chromatography (HPLC) was employed to analyze the flowers and phyllodes. A total of 13 compounds (accounting for most abundant compounds in flowers and phyllodes) include different flavonoids and phenolic compounds. The phytochemical compounds detected were: Gallic acid, protocatechuic acid, -hydroxybenzoic acid, -hydroxybenzaldehyde, vanillic acid, syringic acid, -coumaric acid, and ferulic acid. The major flavonoid compounds identified include rutin, luteolin, apigenin, and catechin. Allelopathic effects of flower and phyllodes extracts from may be due to the presence of above compounds identified by HPLC analysis.
我们研究了毛相思子地上部分枝叶水提取物（0%、25%、50%、75%和100%）对通用生物测试物种生菜幼苗生长和植物生物量的多酚（酚类化合物和黄酮类化合物）组成及化感作用。暴露于地上部分枝叶、花水提取物（AFE）和叶状柄水提取物（APE）6天后，平均叶鲜重、叶干重、根鲜重和根干重均下降。用AFE处理后，植物干生物量减少超过50%。分别用浓度为75%和100%的花提取物（AFE）处理后，平均根长度减少约37.7%和29.20%。花提取物和叶状柄提取物均使生菜根干重降低。地上部分枝叶提取物处理后生菜叶片蛋白质含量降低，这证明AFE和APE均含有导致毒性的多酚，从而致使叶片蛋白质含量下降。采用高效液相色谱法（HPLC）分析花和叶状柄。总共13种化合物（占花和叶状柄中最丰富的化合物）包括不同的黄酮类化合物和酚类化合物。检测到的植物化学化合物有：没食子酸、原儿茶酸、对羟基苯甲酸、对羟基苯甲醛、香草酸、丁香酸、对香豆酸和阿魏酸。鉴定出的主要黄酮类化合物包括芦丁、木犀草素、芹菜素和儿茶素。毛相思子花和叶状柄提取物的化感作用可能归因于HPLC分析鉴定出的上述化合物的存在。
