Li Yu, Wang Xue, Zhang Hao, Ye Xiangsheng, Shi Lei, Xu Fangsen, Ding Guangda
College of Resources and Environment, Microelement Research Center, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.
Plants (Basel). 2023 Sep 22;12(19):3362. doi: 10.3390/plants12193362.
Inorganic phosphate (Pi) is actively taken up by Pi transporters (PTs) from the soil and transported into the plant. Here, we functionally characterized the gene which belongs to the PHT1 family. BnaPT37 is a plasma membrane-localized protein containing 534 amino acids. Expression of increased significantly under Pi deficiency in various tissues, especially in fully expanded leaves. Expression of the β-glucuronidase reporter gene driven by the promoter showed that is expressed in the root, stem, calyx, and leaf under Pi deficiency. can complement a yeast mutant strain defective in five Pi transporters and can restore the growth of the double mutant under Pi deprivation. Overexpression of in rapeseed significantly increased Pi translocation from root to shoot. Moreover, the movement of Pi from fully expanded leaves to new leaves and roots was enhanced in the transgenic lines compared to the wild type. However, the overexpression of inhibited the flowering time, plant height, and Pi accumulation in seeds. In conclusion, BnaPT37 functions as a plasma membrane-localized Pi transporter and might be involved in Pi translocation from root to shoot and Pi distribution from source to sink in .
无机磷酸盐（Pi）由Pi转运蛋白（PTs）从土壤中主动吸收并转运到植物体内。在此，我们对属于PHT1家族的基因进行了功能鉴定。BnaPT37是一种定位于质膜的蛋白质，含有534个氨基酸。在各种组织中，尤其是在完全展开的叶片中，Pi缺乏时其表达显著增加。由该启动子驱动的β-葡萄糖醛酸酶报告基因的表达表明，在Pi缺乏时，其在根、茎、花萼和叶片中表达。它可以互补在五个Pi转运蛋白上有缺陷的酵母突变菌株，并能在Pi缺乏条件下恢复该双突变体的生长。在油菜中过表达它显著增加了Pi从根到地上部的转运。此外，与野生型相比，转基因株系中Pi从完全展开的叶片向新叶和根的移动增强。然而，过表达它会抑制开花时间、株高以及种子中的Pi积累。总之，BnaPT37作为一种定位于质膜的Pi转运蛋白，可能参与了Pi从根到地上部的转运以及油菜中Pi从源到库的分配。
