College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
Hospital of Stomatology, Jilin University, Changchun 130015, China.
Molecules. 2023 Sep 30;28(19):6887. doi: 10.3390/molecules28196887.
Antler ossified tissue has been widely used for the extraction of bioactive peptides. In this study, collagen was prepared from antler ossified tissue via acetic acid and pepsin. Five different proteases were used to hydrolyze the collagen and the hydrolysate treated by neutrase (collagen peptide named ACP) showed the highest DPPH radical clearance rate. The extraction process of ACP was optimized by response surface methodology, and the optimal conditions were as follows: a temperature of 52 °C, a pH of 6.1, and an enzyme concentration of 3200 U/g, which resulted in the maximum DPPH clearance rate of 74.41 ± 0.48%. The peptides (ACP-3) with the strongest antioxidant activity were obtained after isolation and purification, and its DPPH free radical clearance rate was 90.58 ± 1.27%; at the same time, it exhibited good scavenging activity for ABTS, hydroxyl radical, and superoxide anion radical. The study investigated the protective effect of ACP-3 on oxidative damage in HaCaT cells. The findings revealed that all groups that received ACP-3 pretreatment exhibited increased activities of SOD, GSH-Px, and CAT compared to the model group. Furthermore, ACP-3 pretreatment reduced the levels of ROS and MDA in HaCaT cells subjected to HO-induced oxidative damage. These results suggest that collagen peptides derived from deer antler ossified tissue can effectively mitigate the oxidative damage caused by HO in HaCaT cells, thereby providing a foundation for the utilization of collagen peptides in pharmaceuticals and cosmetics.
鹿角骨化组织已被广泛用于提取生物活性肽。本研究通过醋酸和胃蛋白酶从鹿角骨化组织中制备胶原蛋白。五种不同的蛋白酶被用于水解胶原蛋白，经中性蛋白酶处理的水解产物（胶原蛋白肽命名为 ACP）表现出最高的 DPPH 自由基清除率。ACP 的提取工艺通过响应面法进行了优化，最佳条件为：温度 52°C，pH 值 6.1，酶浓度 3200 U/g，此时 DPPH 自由基清除率达到 74.41±0.48%。经过分离纯化得到具有最强抗氧化活性的肽（ACP-3），其 DPPH 自由基清除率为 90.58±1.27%；同时，它对 ABTS、羟自由基和超氧阴离子自由基也表现出良好的清除活性。本研究探讨了 ACP-3 对 HaCaT 细胞氧化损伤的保护作用。结果表明，与模型组相比，所有接受 ACP-3 预处理的组的 SOD、GSH-Px 和 CAT 活性均有所增加。此外，ACP-3 预处理降低了 HO 诱导的氧化损伤后 HaCaT 细胞中 ROS 和 MDA 的水平。这些结果表明，鹿鹿角骨化组织来源的胶原蛋白肽可有效减轻 HO 引起的 HaCaT 细胞氧化损伤，为胶原蛋白肽在药物和化妆品中的应用提供了基础。
