种子提取物对人胃癌细胞系 SNU-1 诱导早期凋亡反应和选择性抗集落形成活性。
Seed Extracts Trigger an Early Apoptosis Response and Selective Anticlonogenic Activity against the Human Gastric Carcinoma Cell Line SNU-1.
机构信息
Laboratorio de Silvigenómica y Biotecnología, Departamento de Biología, Facultad de Ciencias, Universidad de La Serena, Avenida Raúl Bitrán 1305, Casilla 599, La Serena 1700000, Chile.
Facultad de Ciencias Agropecuarias, Universidad del Alba, Cuatro Esquinas 060, La Serena 1700000, Chile.
出版信息
Molecules. 2023 Oct 2;28(19):6906. doi: 10.3390/molecules28196906.
The aim of this study was to evaluate, for the first time, the antiproliferative, apoptotic and diminishing effects of the anchored growth-independent capacity of an ethanol macerate extract from the seed (EMCHS) in the human gastric cancer cell line SNU-1. The cells treated with EMCHS (20 μg/mL) significantly reduced the capacity to form clones of the tumor cell. Moreover, 50 μg/mL of EMCHS extract induced apoptosis, as was shown by the Annexin-V assay. UHPLC-MS/MS analysis detected two acetogenins ( and ) in the EMCHS, which could be largely responsible for its selective antiproliferative effect. The identification of fatty acids by GC-FID showed the presence of eight fatty acids, among which was, oleic acid, which has recognized activity as an adjuvant in antitumor treatments. Taken together, our results indicate that the EMCHS seems promising for use as a natural therapy against gastric cancer disease.
本研究旨在首次评估种子乙醇提取物（EMCHS）的锚定生长独立性对人胃癌细胞系 SNU-1 的增殖、凋亡和减少作用。用 EMCHS（20μg/ml）处理的细胞显著降低了肿瘤细胞形成克隆的能力。此外，50μg/ml 的 EMCHS 提取物通过 Annexin-V 分析诱导细胞凋亡。UHPLC-MS/MS 分析在 EMCHS 中检测到两种乙酰氧基化合物（和），这可能是其选择性增殖抑制作用的主要原因。GC-FID 分析鉴定的脂肪酸表明存在八种脂肪酸，其中包括具有作为抗肿瘤治疗佐剂的公认活性的油酸。总之，我们的结果表明，EMCHS 似乎有望作为一种天然疗法用于治疗胃癌。
Zotero 插件