Laboratorio de Silvogenómica y Biotecnología, Departamento de Biología, Facultad de Ciencias Universidad de La Serena, Avenida Raúl Bitrán 1305, Casilla 599, 1700000, La Serena, Chile.
Laboratorio de Biología Molecular y Celular del Cáncer, Departamento de Ciencias Biomédicas, Facultad de Medicina, Universidad Católica del Norte, Larrondo 1281, 1781421, Coquimbo, Chile.
Plant Foods Hum Nutr. 2019 Sep;74(3):322-327. doi: 10.1007/s11130-019-00742-w.
Annona cherimola is a tree belonging to the family Annonacea, whose fruit (cherimoya) is very desirable, but its seeds are considered waste. Present in these seeds are compounds that have been described as selective antiproliferative agents for cancer cells. The aim of this study was to evaluate the antiproliferative activity of ethanol macerate extract (EMCHS) obtained from A. cherimola seeds against the human stomach gastric adenocarcinoma (AGS) cell line and the normal human gastric epithelial cell line (GES-1). The EMCHS extract presented an IC of 80.43 μg/mL in AGS cells, and a selectivity index (SI) of 3.5-fold higher than that of cisplatin. In addition, the EMCHS extract showed apoptotic activity in AGS cells since 50 μg/mL. Overxpression of PUMA gene in both cells demonstrate that EMCHS activate the apoptotic route. Future studies should be carried out to elucidate anticancer activity of EMCHS in vivo. This work represents the first showing antiproliferative effects of crude extracts obtained from seeds of A. cherimola in AGS cells.
番荔枝是番荔枝科的一种树，其果实（番荔枝）非常受欢迎，但种子被认为是废物。这些种子中含有一些化合物，被描述为癌细胞的选择性增殖抑制剂。本研究旨在评估从番荔枝种子中获得的乙醇浸膏提取物（EMCHS）对人胃腺癌细胞系（AGS）和正常人类胃上皮细胞系（GES-1）的增殖抑制活性。EMCHS 提取物在 AGS 细胞中的 IC 为 80.43μg/mL，其选择性指数（SI）比顺铂高 3.5 倍。此外，EMCHS 提取物在 50μg/mL 时在 AGS 细胞中表现出凋亡活性。在两种细胞中过表达 PUMA 基因表明，EMCHS 激活了凋亡途径。未来的研究应阐明 EMCHS 在体内的抗癌活性。这项工作首次表明，从番荔枝种子中获得的粗提取物对 AGS 细胞具有增殖抑制作用。
